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Documentos clave

G1924

Sigma-Aldrich

L-Glutamate Oxidase from Streptomyces sp.

recombinant, expressed in E. coli

Sinónimos:

L-Glutamate:oxygen oxidoreductase (deaminating)

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About This Item

Número de CAS:
39346-34-4
MDL number:
MFCD00131459
UNSPSC Code:
12352204
NACRES:
NA.54
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recombinant

expressed in E. coli

Quality Level

200

form

solid

specific activity

≥5 units/mg protein

mol wt

120 kDa

storage temp.

−20°C

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General description

L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa.[1] The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.[2]
This product has a 6xHistidine tag with a thrombin cleavage site.

Application

L-Glutamate Oxidase from Streptomyces sp may be used:
  • as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development[3]
  • as a component of pencil graphite electrode for biosensing L-glutamate[4]

Biochem/physiol Actions

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide.[1] LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.[2]

Unit Definition

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000356452
0000356451
0000356452
0000356451
0000230084

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Isolation, purification, and characterization of L-glutamate oxidase from Streptomyces sp. 18G
Wachiratianchai S, et al.
Electronic journal of Biotechnology, 7(3), 09-10 (2004)
L-Glutamate biosensor based on l-glutamate oxidase immobilized onto ZnO nanorods/polypyrrole modified pencil graphite electrode
Batra B, et al.
Biochemical Engineering Journal, 105, 428-436 (2016)
Comparative study of hydrogel-immobilized L-glutamate oxidases for a novel thick-film biosensor and its application in food samples
Kwong AWK, et al.
Biotechnology Letters, 22(4), 267-272 (2000)
Jiro Arima et al.
The FEBS journal, 276(14), 3894-3903 (2009-06-18)
L-Glutamate oxidase (LGOX) from Streptomyces sp. X-119-6, which catalyzes the oxidative deamination of L-glutamate, has attracted increasing attention as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry. The precursor of LGOX, which has a
Andrea C Kneuttinger et al.
Cell chemical biology, 26(11), 1501-1514 (2019-09-10)
Imidazole glycerol phosphate synthase (ImGPS) is an allosteric bienzyme complex in which substrate binding to the synthase subunit HisF stimulates the glutaminase subunit HisH. To control this stimulation with light, we have incorporated the photo-responsive unnatural amino acids phenylalanine-4'-azobenzene (AzoF)

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