AL0103000
ALiCE® Cell Free Protein Expression Kit (Plant Cell Lysate)
An eukaryotic cell free expression system with unprecedented 3 mg/mL recombinant protein yield in batch mode and post-translational modifications enabled
Sinónimos:
Cell-Free Expression Kit
About This Item
Productos recomendados
form
liquid (aqueous solutions)
packaging
pkg of 6 × 200 μL (AL0103200 (ALiCE® Midi Kit))
pkg of 6 × 50 μL (AL0103050 (ALiCE® Mini Kit))
pkg of 6 × 500 μL (AL0103500 (ALiCE® Maxi Kit))
technique(s)
protein expression: suitable
shipped in
dry ice
storage temp.
−70°C
General description
To address this problem, ALiCE® a novel eukaryotic cell-free expression system was created by taking advantage of plant cell biology. ALiCE® offers unprecedented yields of up to 3 mg/mL of protein in batch mode. As such, it enables you to overcome the limitations of living host systems and to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
ALiCE® utilizes tobacco cell lysates, depleted of vacuoles. The system contains all factors required for in vitro transcription (RNA polymerases, NTPs) and translation reactions (ribosomes, translation initiation/elongation factors, tRNA, etc.). Using a simple protocol, both the RNA transcription and translation take place in a single tube (coupled transcription/translation).
Reactions are started by simply mixing plasmid DNA with the ALiCE® Reaction mix. The outstanding protein yields of ALiCE® are based on highly efficient protein expression over the duration of the reaction. Consequently, we recommend a reaction duration of 48 h.
ALiCE® is provided with two expression vectors, pALiCE01 and pALiCE02, which are used according to the targeting of the protein of interest. These have been specially tailored to offer excellent protein yield when used with the ALiCE® expression system. pALiCE01 is used for expression in ALiCE® cytosolic fraction. Targeting the microsomes with pALiCE02 offers the opportunity to express protein with post-translational modifications like disulfide bonds.
Application
- Target protein characterization
- Protein optimization
- Mutant screening
- Protein-protein interactions
- Expression analysis
- Protein localization analysis
- Protein structure analysis
- Post-translational modification assays
- Metabolomics
- Herbicide screening
Features and Benefits
- High-yield: Up to 3 mg/mL expressed protein
- Easy to use: One-step reaction protocol
- Fast: High yield in just 48 hours
- Flexible: Express “difficult to produce” proteins, cytotoxic proteins, etc.
Components
ALiCE® Lysate, 6 x 50 μL
Vector pALiCE01, 15 μL
Vector pALiCE02, 15 μL
ALiCE® Tube Cap, 0.6 mm hole
ALiCE® Midi Kit - AL0103200 Comprises
ALiCE® Lysate, 6 x 200 μL
Vector pALiCE01, 50 μL
Vector pALiCE02, 50 μL
ALiCE® Tube Set, 12 pcs
ALiCE® Maxi Kit - AL0103500 Comprises
ALiCE® Lysate, 6 x 500 μL
Vector pALiCE01, 50 μL
Vector pALiCE02, 50 μL
ALiCE® Tube Set, 12 pcs
Storage and Stability
- ALiCE® Reaction (Lysate) mix: store at -70 °C
- Vector pALiCE01: store at -20 °C to -70 °C
- Vector pALiCE02: store at -20 °C to -70 °C
- ALiCE® Tube caps/Tube set: store at room temperature
Legal Information
Storage Class
10 - Combustible liquids
wgk_germany
WGK 3
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Cell-free protein synthesis rapidly produces recombinant proteins without cells, utilizing translation machinery from different cell types for efficiency.
Cell-free protein synthesis rapidly produces recombinant proteins without cells, utilizing translation machinery from different cell types for efficiency.
Cell-free protein synthesis rapidly produces recombinant proteins without cells, utilizing translation machinery from different cell types for efficiency.
Cell-free protein synthesis rapidly produces recombinant proteins without cells, utilizing translation machinery from different cell types for efficiency.
Protocolos
Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.
Contenido relacionado
Read the Limited Use Label License that details how the ALiCE™ Cell Free Protein Expression System is available for in vitro research use only.
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
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