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A9046

Sigma-Aldrich

Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti Chicken Igy

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About This Item

MDL number:
MFCD00162225
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

peroxidase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

chicken

technique(s)

direct ELISA: 1:30,000
dot blot: 1:160,000 (indirect chemiluminescence)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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General description

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgG.

Immunogen

purified chicken IgG

Application

Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody produced in rabbit has been used in indirect enzyme-linked immunosorbent assay (ELISA), dierct ELISA, dot blot and immunohistochemistry.
Proteins isolated from rabbit glomeruli by iron oxide magnetization were subjected to western blot analysis using HRP-conjugated rabbit anti-chicken IgY as the secondary antibody.
Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for immunoprecipitation and western blot (1:2,000) assays. The antibody can also be used for direct ELISA (1:30,000), dot blot (1:12,000-1:15,000) and IHC (1:1,000) applications.

Biochem/physiol Actions

Egg yolk IgY immunoglobulin acquired from hen have been studied extensively. It is useful in immunological assays and does interact with the rheumatoid factor.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Yasmina Noubia Abdiche et al.
mAbs, 8(2), 264-277 (2015-12-15)
The ability of monoclonal antibodies (mAbs) to target specific antigens with high precision has led to an increasing demand to generate them for therapeutic use in many disease areas. Historically, the discovery of therapeutic mAbs has relied upon the immunization
Antal Nyeste et al.
The Journal of biological chemistry, 291(9), 4473-4486 (2016-01-02)
The prion protein (PrP) seems to exert both neuroprotective and neurotoxic activities. The toxic activities are associated with the C-terminal globular parts in the absence of the flexible N terminus, specifically the hydrophobic domain (HD) or the central region (CR).
Production and purification of IgY antibodies from chicken egg yolk.
Amro W A, et al.
Journal of Genetic Engineering and Biotechnology, 16(1), 99-103 (2018)
Frequency of anti-Toxocara antibodies in broiler chickens in southern Brazil
Oliveira A, et al,
Revista Brasileira de Parasitologia veterinaria = Brazilian Journal of Veterinary Parasitology : Org?o Oficial do Colegio Brasileiro de Parasitologia Veterinaria, 27(2), 141-145 (2018)
Germline polymorphisms and survival of lung adenocarcinoma patients: A genome-wide study in two European patient series
Galvan A, et al.
International Journal of Cancer. Journal International Du Cancer, 136(5), E262-E271 (2015)
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