Yes, this product is stable until recommended retest date in original container. This product is a prepared solution of 1M DTT in water. In the original sealed container it is quite stable, with an assigned retest schedule of every 2 years. Assuming that the product is handled properly, the material is expected to be stable until at least the retest date reported on the lot specific Certificate of Analysis.
43816
DL-Ditiotreitol solution
BioUltra, Molecular Biology, ~1 M in H2O
Sinónimos:
Reactivo de Cleland′, (±)-ditiotreitol, rac-ditiotreitol, ditiotreitol, (±)-treo-1,4-Dimercapto-2,3-butanediol solution, DTT
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Fórmula empírica (notación de Hill):
C4H10O2S2
Número CAS:
Peso molecular:
154.25
UNSPSC Code:
12352201
NACRES:
NA.25
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1719757
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Molecular Biology
Quality Segment
product line
BioUltra
assay
900-1100 mmol/L (Titration molarity)
form
liquid
reaction suitability
reagent type: reductant
concentration
~1 M in H2O
impurities
DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
pH
2.7-5.0
density
1.04 g/mL at 20 °C
absorption
cut-off at 330 nm
suitability
in accordance for filter test, suitable for molecular biology
storage temp.
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
General description
El ditiotreitol (TDT), un protector del grupo tiol,[1] es un excelente reactivo para evitar la oxidación de los grupos SH en las proteínas. La disolución de TDT es un agente reductor de disulfuros potente y versátil.[2] Reduce un enlace disulfuro formando un anillo de seis miembros e iniciando un intercambio tiol-disulfuro.
Application
La disolución de Dl-ditiotreitol-ditiotreitol se ha utilizado :
- en el tratamiento de los explantes intestinales
- en la preparación de homogeneizados de células branquiales para ensayos enzimáticos
- en electroforesis de proteínas para inmunoelectrotransferencia
- en actividad transglutaminasa
- tampón de muestras para SDS-PAGE
- en Western Blot, como componente del tampón de lisis para la secuenciación de ARN de un solo núcleo
- como componente del tampón de extracción de proteínas para lisis celular
Biochem/physiol Actions
El DL-ditiotreitol (DTT) se utiliza en una técnica de desunión química, que ayuda a quitar las bacterias de las biopelículas. Puede cambiar la estabilidad estructural del receptor GABA(B) (ácido gamma-aminobutírico) al desactivar los enlaces disulfuro entre cuatro restos de cisteína situados en la estructura del receptor GABA(B1(a)).El ditiotreitol (TDT) se utiliza mucho en la síntesis peptídica sintética y la reordenación bioquímica de las tiolproteínas. También se puede utilizar para estudiar temas de química de proteínas como la actividad enzimática y el plegamiento de proteínas. El DTT controla explícitamente la respuesta de intercambio tiol-disulfuro para reducir por completo los enlaces disulfuro subatómicos intra o inter en las biomoléculas. Los tioles y el disulfuro cíclico del DTT son los productos de esta reacción.
Features and Benefits
- Calidad BioUltra - Disolución de DTT 1M, adecuada para biología molecular
- Sin ADNasas, ARNasas, fosfatasas y proteasas
Other Notes
Si desea más información sobre nuestra gama de productos bioquímicos, cumplimente este formulario.
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Este artículo | |||
|---|---|---|---|
| grade Molecular Biology | grade - | grade Molecular Biology | grade Molecular Biology |
| assay 900-1100 mmol/L (Titration molarity) | assay - | assay ≥99.5% (RT) | assay ≥98% (HPLC), ≥99% (titration) |
| product line BioUltra | product line - | product line BioUltra | product line - |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level 200 |
| impurities DNases, none detected, phosphatases, none detected, RNases, none detected, proteases, none detected | impurities - | impurities DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.3% 4,5-dihydroxy-1,2-dithiane | impurities - |
| storage temp. 2-8°C | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
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Clase de almacenamiento
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Número de artículo de comercio global
| SKU | GTIN |
|---|---|
| 43816-50ML | 04061838092311 |
| 43816-250ML | 04061832132419 |
| 43816-10ML | 04061832132334 |
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After opening DTT ref 43816-50ML, is the product stable? Can it be reused?
1 answer-
Helpful?
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How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
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How stable is this DTT solution ? it is known that DTT is unstable in solution and should be kept under inert atmosphere. How long can I keep it once open ?
1 answer-
This product is a prepared solution of 1M DTT in water. In the original sealed container it is quite stable, with an assigned retest schedule of every 2 years. Assuming that the product is handled properly, the material is expected to be stable until at least the retest date reported on the lot specific Certificate of Analysis.
Helpful?
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