I9274

Invertase from baker′s yeast (S. cerevisiae)

200-300 units/mg solid

• Sinónimos: β-D-Fructofuranosidase, β-D-Fructofuranoside fructohydrolase, Saccharase
• Número de CAS: 9001-57-4
• Comisión internacional de enzimas: 3.2.1.26 (BRENDA, IUBMB)
• Número CE: 232-615-7
• Número MDL: MFCD00131410
• Código UNSPSC: 12164500
• NACRES: NA.21

Propiedades

• Nivel de calidad: 100
• actividad específica: 200-300 units/mg solid
• técnicas: photometry: suitable
• aplicaciones: food and beverages; general analytical
• actividad extraña: α-glucosidase (maltase), phosphoglucomutase, phosphoglucose isomerase ≤0.01%; 6-phosphogluconic dehydrogenase, α-galactosidase, β-galactosidase, and NADH oxidase ≤0.001%
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Invertase is encoded by the SUC2 (Saccharomyces cerevisiae S288C) gene. In yeast this enzyme exist in: glycosylated (secreted) and non-glycosylated (intracellular) form. The external invertase is composed of carbohydrate (50%) and 60,000 D subunits.

Aplicación

Invertase from baker′s yeast (S. cerevisiae) has been used:

• in liquid media (YES) and is used to determine the invertase activity
• supplemented to the reaction mixture in the extraction and analysis of sugars and starch
• for sucrose hydrolysis

Used in the production of confectionary foods and artificial honey.

Acciones bioquímicas o fisiológicas

Invertase hydrolyzes sucrose into glucose and fructose yielding a colorless product, unlike acid hydrolysis which produces colored products.

Definición de unidad

One unit will hydrolyze 1.0 μmole sucrose to glucose and fructose per minute at pH 4.6 at 25 °C.

Información de seguridad

• Pictogramas: GHS08
• Palabra de señalización: Danger
• Frases de peligro: H334
• Consejos de prudencia: P261 - P284 - P501
• Clasificaciones de peligro: Resp. Sens. 1
• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)