MABE911

Anti-Aiolos Antibody, clone 9D10

clone 9D10, from mouse

• Sinónimos: Zinc finger protein Aiolos, Ikaros family zinc finger protein 3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propiedades

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9D10, monoclonal
• species reactivity: human, mouse
• technique(s): ChIP: suitable (ChIP-seq); western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_001244337
• UniProt accession no.: Q9UKT9
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... IKZF3(22806)

Descripción

General description

Aiolos is a zinc finger DNA binding transcription factor important in lymphocyte differentiation and development. Aiolos plays an essential role in the regulation of B-cell differentiation including, proliferation and maturation to plasma cells. Aiolos also works as a cofactor in gene suppression via its interaction with the transcription factor FOXP3 in regulatory T cells as well, and Aiolos is involved in various chromatin functions too through its interaction with histone deacetylase complexes containing HDAC1, MTA2 and SIN3A. Aiolos is localized in the nucleus primarily but can be found in the cytoplasm depending upon the cellular situation. Aiolos is expressed in leukocytes and found in high concentration in spleen and thymus and has multiple isoforms. Activity is regulated via phosphorylation.

Immunogen

Recombinant protein corresponding to human Aiolos.

Application

Chromatin Immunoprecipitation (ChIP): A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).

ChIP-seq: A representative lot of this antibody was reported to work in ChIP (see Zhang, J., et al 2012).

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in an HL-60 cell lysate

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

This Anti-Aiolos Antibody, clone 9D10 is validated for use in Western Blotting and ChIP-seq and Chromatin Immunoprecipitation (ChIP) for the detection of Aiolos.

Quality

Evaluated by Western Blotting in a Jurkat cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected Aliolos in 10 µg of a Jurkat cell lysate.

Target description

~ 58-70 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable