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Key Documents

MAB16990

Sigma-Aldrich

Anti-Hepatitis B Virus Antibody, core Antigen (ayw), clone 14E11, a.a. 134-140

ascites fluid, clone 14E11, Chemicon®

Sinónimos:

HBcAg

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

300

antibody form

ascites fluid

antibody product type

primary antibodies

clone

14E11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2b

shipped in

wet ice

Specificity

Reacts with HBcAg (epitope recognized - amino acid positions 135-140).

SPECIES REACTIVITIES:

Cross-reacts with woodchuck hepatitis virus core antigen (WHcAg), but not with duck hepatitis B virus core antigen (DHBcAg).

Immunogen

Epitope: Core Antigen (ayw)
Recombinant purified HBcAg from E.coli, SDS-denatured

Application

ELISA, immunoblotting, immunofluorescent and immunoperoxidase staining of infected liver tissues and transfected cultured cells.

Recommended working dilutions: for immunoblotting - 1:1000; for immunofluorescence - 1:200. Optimal working dilutions must be determined by the end user.
Research Category
Infectious Diseases
Research Sub Category
Infectious Diseases - Viral
This Anti-Hepatitis B Virus Antibody, core antigen (ayw), clone 14E11, a.a. 134-140 is validated for use in ELISA, IF, IP, WB, IC, IH for the detection of Hepatitis B Virus.

Physical form

Ascites. Liquid, containing 0.1% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Immunochemical structure of the carboxy-terminal part of hepatitis B e antigen: identification of internal and surface-exposed sequences
Sallberg, M, et al
The Journal of General Virology, 74 ( Pt 7), 1335-1340 (1993)
Boerries Brandenburg et al.
Hepatology (Baltimore, Md.), 42(6), 1300-1309 (2005-12-01)
Protein transduction domains (PTDs) have been used to deliver a variety of biologically active cargo across cellular membranes. However the potential of PTDs to mediate transport of nanoparticular structures into the cytoplasm bypassing the endosomal compartment remains unclear. Cell-permeable virus-like
Tobias Zahn et al.
Vaccine, 38(15), 3105-3120 (2020-03-03)
There are currently about 257 million people suffering from chronic HBV infection worldwide. In many cases, an insufficient Tcell response is causative for establishment of a chronic infection. To ensure a robust cellular immune response and induction of neutralizing antibodies
The affinities of monoclonal antibodies against core antigen of hepatitis B virus.
Apsalons, U and Bichko, V
Archives of Virology, 134, 393-402 (1994)
V Bichko et al.
Molecular immunology, 30(3), 221-231 (1993-02-01)
Particulate and denatured core protein as well as e-antigen (HBe) of hepatitis B virus (HBV) differ in part immunologically but this has not been studied in sufficient detail. Therefore, in this study the B-cell immune response to native and denatured
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