444203

2- Mercaptoetanol

A disulfide-reducing agent.

• Sinónimos: β-Mercaptoetanol, 2- Hidroxietilmercaptano, BME, Tioetilenglicol
• Fórmula lineal: HSCH₂CH₂OH
• Número de CAS: 60-24-2
• Peso molecular: 78.13
• Beilstein/REAXYS Number: 773648
• MDL number: MFCD00004890
• UNSPSC Code: 12161504
• NACRES: NA.25

Propiedades

• grade: Molecular Biology
• Quality Level: 100
• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• assay: ≥99% (GC)
• form: liquid
• expl. lim.: 18 %
• reaction suitability: reagent type: reductant
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• concentration: 14.3 M (pure liquid)
• refractive index: n20/D 1.500 (lit.)
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• mp: <-50 °C
• density: 1.12 g/cm³ at 20 °C; 1.114 g/mL at 25 °C (lit.)
• cation traces: heavy metals (as Pb): ≤0.0001%
• absorption: ≤0.31 at 280 nm; ≤1.50 at 260 nm
• foreign activity: DNases, proteases, RNases, none detected
• shipped in: ambient
• storage temp.: 15-25°C
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Descripción

General description

A disulfide-reducing agent.

A disulfide-reducing agent. Reagent commonly used in organic synthesis (aborsorbance: ≤1.50 at 260 nm; ≤0.31 at 280 nm).

Application

BME es idóneo para reducir los puentes disulfuro de las proteínas antes de la electroforesis en gel de poliacrilamida y suele incluirse en un tampón de muestra para SDS-PAGE a una concentración de un 5 %. La escisión de los puentes disulfuro intermoleculares (entre subunidades) permite que las subunidades de una proteína se separen de forma independiente en la SDS-PAGE. La escisión de los puentes disulfuro intramoleculares (dentro de la subunidad) permite la desnaturalización completa de las subunidades para que cada péptido migre en función de la longitud de su cadena sin influencia de la estructura secundaria.

Warning

Toxicity: Toxic (F)

Other Notes

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Información de seguridad

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• pcodes: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• target_organs: Liver,Heart
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3
• flash_point_f: 165.2 °F - closed cup
• flash_point_c: 74 °C - closed cup