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777323

Sigma-Aldrich

Cy5-azide

95% (HPLC)

Sinónimos:

Cy5 Azide Fluorescent Probe

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About This Item

Fórmula empírica (notación de Hill):
C49H78N8O10S3
Peso molecular:
1035.39
UNSPSC Code:
12352125
PubChem Substance ID:
329767899
NACRES:
NA.22
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Quality Level

100

assay

95% (HPLC)

form

solid

reaction suitability

reaction type: click chemistry

ε (extinction coefficient)

251,000

fluorescence

λex 647 nm; λem 663 nm

storage temp.

−20°C

SMILES string

CC1(C)C(/C=C/C=C/C=C(N2CCCS([O-])(=O)=O)\C(C)(C)C3=C2C=CC(S([O-])(=O)=O)=C3)=[N+](CCCCCC(NCCCN=[N+]=[N-])=O)C4=C1C=C(S([O-])(=O)=O)C=C4.CC[NH+](CC)CC.CC[NH+](CC)CC

InChI

1S/C37H48N6O10S3.2C6H15N/c1-36(2)29-25-27(55(48,49)50)16-18-31(29)42(22-10-6-9-15-35(44)39-20-11-21-40-41-38)33(36)13-7-5-8-14-34-37(3,4)30-26-28(56(51,52)53)17-19-32(30)43(34)23-12-24-54(45,46)47;2*1-4-7(5-2)6-3/h5,7-8,13-14,16-19,25-26H,6,9-12,15,20-24H2,1-4H3,(H3-,39,44,45,46,47,48,49,50,51,52,53);2*4-6H2,1-3H3

InChI key

NJSWGOLBDDXJQO-UHFFFAOYSA-N

Application

Azide modified cyanine dye that can be used to detect or label alkyne- or cyclooctyne-containing molecules or biomolecules by fluorescence spectroscopy following azide-alkyne cycloaddition.

Solubility: DMSO, DMF, MeOH

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
MKCW7635
MKCV9501
MKCR9311
MKCQ7519
MKCR4683

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Chemical tools to monitor drug-target engagement of endogenously expressed protein kinases are highly desirable for preclinical target validation in drug discovery. Here, we describe a chemical genetics strategy to selectively study target engagement of endogenous kinases. By substituting a serine
Shanqiao Wei et al.
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Australian journal of chemistry, 73(4), 327-333 (2020-05-13)
In-solution conjugation is the most commonly used strategy to label peptides and proteins with fluorophores. However, lack of site-specific control and high costs of fluorophores are recognised limitations of this approach. Here, we established facile access to grams of Cy5-COOH
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Bioconjugate chemistry, 28(10), 2599-2607 (2017-09-19)
We developed a novel technique for the efficient conjugation of oligonucleotides with various alkyl azides such as fluorescent dyes, biotin, cholesterol, N-acetylgalactosamine (GalNAc), etc. using copper-catalysed alkyne-azide cycloaddition on the solid phase and CuI·P(OEt)
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Questions

  1. Hi, I am trying to conjugate cy5 to a protein already has azide at its C-terminus. What would the protocol would be in this case? Do we need to use CuAAC in our case?

    1 answer

    1. This product is a Cy5-azide. Conjugation may not be possible if the protein of interest has a terminal azide rather than a terminal alkyne. Should this be the case, a Cy5-alkyne such as product 777358, would be recommended. Click chemistry relies on 1,3-dipolar cycloaddition of alkynes to azides to form 1,4-disubsituted-1,2,3-triazoles.
      Please see the link below to review an introduction to chemistry:
      https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/chemistry-and-synthesis/adc-and-bioconjugation/click-chemistry

      Please see the link below to review product 777358, Cy5-alkyne:
      https://www.sigmaaldrich.com/product/aldrich/777358

      A search of the scientific literature may be carried out to develop the intended application. The suggested key terms of interest may be azide, alkyne, and click chemistry.

      Helpful?

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