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  • Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile.

Electrospraying technique for the fabrication of metronidazole contained PLGA particles and their release profile.

Materials science & engineering. C, Materials for biological applications (2015-08-08)
Molamma P Prabhakaran, Maedeh Zamani, Betiana Felice, Seeram Ramakrishna
ABSTRACT

Advanced engineering of materials for the development of drug delivery devices provides scope for novel and versatile strategies for treatment of various diseases. 'Electrospraying' was used to prepare PLGA microparticles and further encapsulate the drug, metronidazole (Met) within the particles to function as a drug delivery system. Two different solvents were utilized for the preparation of drug loaded PLGA particles, whereby the polymeric solution in dichloromethane (DCM) produced particles of bigger sizes than using trifluoroethanol (TFE). Scanning electron microscopy showed the spherical morphology of the particles, with sizes of 3946±407nm and 1774±167nm, respectively for PLGA-Met(DCM) and PLGA-Met(TFE). The FTIR spectroscopy proved the incorporation of metronidazole in the polymer, but without any specific drug-polymer interaction. The release of the drug from the particles was studied in phosphate buffered saline, where a sustained drug release was obtained for at least 41days. Cytotoxicity evaluation of the drug extract using mesenchymal stem cells (MSCs) showed not hindering the proliferation of MSCs, and the cell phenotype was retained after incubation in the drug containing media. Electrospraying is suggested as a cost-effective and single step process for the preparation of polymeric microparticles for prolonged and controlled release of drug.

MATERIALS
Product Number
Brand
Product Description

Supelco
Dichloromethane solution, contains 10 % (v/v) methanol
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Ethylenediaminetetraacetic acid, SAJ special grade, ≥99.0%
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Dichloromethane, SAJ first grade, ≥99.0%
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Dichloromethane, JIS special grade, ≥99.0%
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Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer
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Dichloromethane, anhydrous, ≥99.8%, contains 40-150 ppm amylene as stabilizer
Supelco
Dichloromethane, ≥99.9%
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Ethylenediaminetetraacetic acid, 99.995% trace metals basis
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Ethylenediaminetetraacetic acid, anhydrous, crystalline, BioReagent, suitable for cell culture
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Ethylenediaminetetraacetic acid, purified grade, ≥98.5%, powder
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Ethylenediaminetetraacetic acid, ACS reagent, 99.4-100.6%, powder
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Ethylenediaminetetraacetic acid solution, 0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture
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Ethylenediaminetetraacetic acid, anhydrous, BioUltra, ≥99% (titration)
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Metronidazole, SAJ first grade, ≥99.0%
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Metronidazole, BioXtra