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  • Inhibition of both protease and helicase activities of hepatitis C virus NS3 by an ethyl acetate extract of marine sponge Amphimedon sp.

Inhibition of both protease and helicase activities of hepatitis C virus NS3 by an ethyl acetate extract of marine sponge Amphimedon sp.

PloS one (2012-11-13)
Yuusuke Fujimoto, Kazi Abdus Salam, Atsushi Furuta, Yasuyoshi Matsuda, Osamu Fujita, Hidenori Tani, Masanori Ikeda, Nobuyuki Kato, Naoya Sakamoto, Shinya Maekawa, Nobuyuki Enomoto, Nicole J de Voogd, Masamichi Nakakoshi, Masayoshi Tsubuki, Yuji Sekiguchi, Satoshi Tsuneda, Nobuyoshi Akimitsu, Naohiro Noda, Atsuya Yamashita, Junichi Tanaka, Kohji Moriishi
ABSTRACT

Combination therapy with ribavirin, interferon, and viral protease inhibitors could be expected to elicit a high level of sustained virologic response in patients infected with hepatitis C virus (HCV). However, several severe side effects of this combination therapy have been encountered in clinical trials. In order to develop more effective and safer anti-HCV compounds, we employed the replicon systems derived from several strains of HCV to screen 84 extracts from 54 organisms that were gathered from the sea surrounding Okinawa Prefecture, Japan. The ethyl acetate-soluble extract that was prepared from marine sponge Amphimedon sp. showed the highest inhibitory effect on viral replication, with EC₅₀ values of 1.5 and 24.9 µg/ml in sub-genomic replicon cell lines derived from genotypes 1b and 2a, respectively. But the extract had no effect on interferon-inducing signaling or cytotoxicity. Treatment with the extract inhibited virus production by 30% relative to the control in the JFH1-Huh7 cell culture system. The in vitro enzymological assays revealed that treatment with the extract suppressed both helicase and protease activities of NS3 with IC₅₀ values of 18.9 and 10.9 µg/ml, respectively. Treatment with the extract of Amphimedon sp. inhibited RNA-binding ability but not ATPase activity. These results suggest that the novel compound(s) included in Amphimedon sp. can target the protease and helicase activities of HCV NS3.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ethyl acetate, suitable for HPLC
Sigma-Aldrich
Ethyl acetate, JIS 300, ≥99.5%, for residue analysis
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Ethyl acetate, JIS special grade, ≥99.5%
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Ethyl acetate, SAJ first grade, ≥99.0%
Sigma-Aldrich
Ethyl acetate, JIS 1000, ≥99.5%, for residue analysis
Supelco
Ethyl acetate, analytical standard
Sigma-Aldrich
Ethyl acetate, ≥99%, FCC, FG
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Ethyl acetate, natural, ≥99%, FCC, FG
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Ethyl acetate, anhydrous, 99.8%
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Ethyl acetate, ACS reagent, ≥99.5%
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Ethyl acetate, ReagentPlus®, ≥99.8%
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Ethyl acetate, ACS reagent, ≥99.5%
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Ethyl acetate, ACS reagent, ≥99.5%
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Ethyl acetate, biotech. grade, ≥99.8%
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Ethyl acetate, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99.5% (GC)
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Ethyl acetate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)
Sigma-Aldrich
Ethyl acetate, suitable for HPLC, ≥99.7%
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Ethyl acetate, HPLC Plus, for HPLC, GC, and residue analysis, 99.9%
Sigma-Aldrich
Ethyl acetate, suitable for HPLC, ≥99.8%