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  • Application of several types of substrates to ficin-catalyzed peptide synthesis.

Application of several types of substrates to ficin-catalyzed peptide synthesis.

Amino acids (2007-07-10)
H Sekizaki, E Toyota, T Fuchise, S Zhou, Y Noguchi, K Horita
ABSTRACT

The capability of ficin, a cystine protease, to form peptide bonds was investigated using several types of N-Boc-amino acid phenyl and naphthyl esters as acyl donor components. Enzyme-catalyzed peptide synthesis was carried out under optimized reaction conditions of pH, acyl acceptor concentration and selection of the best yield organic solvent. It used a condensation of N-Boc-Ala-OpGu and Ala-pNA as a model reaction. The products were obtained in 72-96% yield using 10 different substrates, within a few minutes of reaction time.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Ficin from fig tree latex, powder, ≥0.1 unit/mg solid
Sigma-Aldrich
Ficin from fig tree latex, saline suspension, ≥1.0 units/mg protein (biuret)
Sigma-Aldrich
Ficin from fig tree latex, lyophilized powder