Fate of desmosomal proteins in apoptotic epidermal cells.

Fate of desmosomal proteins in apoptotic epidermal cells.

Methods in molecular biology (Clifton, N.J.) (2004-10-27)

Jörg Weiske, Otmar Huber

ABSTRACT

In epidermal cells desmosomes represent major sites of basolateral cell-cell contacts that play an important role for epidermal homeostasis. Excess or damaged cells are often removed by apoptosis. To release apoptotic cells from the tissue, intercellular contacts have to be broken. Here detailed protocols to analyze desmosomal proteins in apoptotic cells are described.

MATERIALS

Product Number

Brand

Product Description

S5921

Sigma-Aldrich

Staurosporine from Streptomyces sp., for molecular biology, ≥95% (HPLC)

C9911

Sigma-Aldrich

(S)-(+)-Camptothecin, ≥90% (HPLC), powder

264155

Sigma-Aldrich

Caspase-3 Inhibitor II, The Caspase-3 Inhibitor II, also referenced under CAS 210344-95-9, controls the biological activity of Caspase-3. This small molecule/inhibitor is primarily used for Cancer applications.

G2500

Sigma-Aldrich

Gelatin from porcine skin, gel strength 300, Type A

G6767

Sigma-Aldrich

Goat Serum Donor Herd, USA origin, sterile-filtered, suitable for cell culture

444250

Sigma-Aldrich

MMP Inhibitor I, The MMP Inhibitor I controls the biological activity of MMP. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.