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Key Documents

SAB1306611

Sigma-Aldrich

ANTI-LC3 (APG8B) (N-TERM) antibody produced in rabbit

purified immunoglobulin, buffered aqueous solution

Synonym(s):

Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1ALC3, MAP1LC3B, Microtubule-associated proteins 1A/1B light chain 3B

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

14688 Da

species reactivity

human, rat

technique(s)

immunofluorescence: 1:100
immunohistochemistry: 1:50-1:100
western blot: 1:1000

NCBI accession no.

NP_073729.1

UniProt accession no.

Q9GZQ8

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAP1LC3B(81631)

General description

The gene LC3B or MAP1LC3B (microtubule associated protein 1 light chain 3 beta) is a homolog of the ATG8 gene, an autophagy-related gene in yeast. The encoded protein is called pro-MAP1LC3B, a pro-form that is cleaved by ATG4 to produce a cytosolic form called MAP1LC3B-I.

Biochem/physiol Actions

MAP1LC3B-I (microtubule associated protein 1 light chain 3 beta-I) conjugates with MAP1LC3B-II before being inserted into both inner and outer membranes of the growing autophagic vesicle during autophagy. The cellular distribution and processing of MAP1LC3B facilitates monitoring of autophagy. Cells that lack MAP1LC3B are found to be defective in the formation of autophagic vesicles, with altered phenotypes associated with the role of autophagy in energy homeostasis and removal of protein aggregates. It can serve as an autophagic marker.

Physical form

Supplied in PBS with 0.09% (W/V) sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The unfolded protein response protects human tumor cells during hypoxia through regulation of the autophagy genes MAP1LC3B and ATG5.
Rouschop KM, et al.
The Journal of Clinical Investigation, 120(1), 127-141 (2010)
Autophagic marker MAP1LC3B expression levels are associated with carotid atherosclerosis symptomatology.
Swaminathan B, et al.
PLoS ONE (2014)
Anna Churilova et al.
International journal of molecular sciences, 23(14) (2022-07-28)
Autophagy is a regulated mechanism of degradation of misfolded proteins and organelles in the cell. Neurons are highly differentiated cells with extended projections, and therefore, their functioning largely depends on the mechanisms of autophagy. For the first time in an

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