Accéder au contenu
Merck

Muscle injury causes long-term changes in stem-cell DNA methylation.

Proceedings of the National Academy of Sciences of the United States of America (2022-12-20)
Tal Falick Michaeli, Ofra Sabag, Rimma Fok, Batia Azria, Jonathan Monin, Yuval Nevo, Yuval Gielchinsky, Benjamin P Berman, Howard Cedar, Yehudit Bergman
RÉSUMÉ

Injury to muscle brings about the activation of stem cells, which then generate new myocytes to replace damaged tissue. We demonstrate that this activation is accompanied by a dramatic change in the stem-cell methylation pattern that prepares them epigenetically for terminal myocyte differentiation. These de- and de novo methylation events occur at regulatory elements associated with genes involved in myogenesis and are necessary for activation and regeneration. Local injury of one muscle elicits an almost identical epigenetic change in satellite cells from other muscles in the body, in a process mediated by circulating factors. Furthermore, this same methylation state is also generated in muscle stem cells (MuSCs) of female animals following pregnancy, even in the absence of any injury. Unlike the activation-induced expression changes, which are transient, the induced methylation profile is stably maintained in resident MuSCs and thus represents a molecular memory of previous physiological events that is probably programmed to provide a mechanism for long-term adaptation.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Anti-KI-67 antibody, Rabbit monoclonal, recombinant, expressed in proprietary host, clone SP6, tissue culture supernatant