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U4002

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type IX, powder, 50,000-100,000 units/g solid

Synonyme(s) :

Jack bean urease, Urea amidohydrolase

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About This Item

Numéro CAS:
9002-13-5
Numéro de classification (Commission des enzymes):
3.5.1.5 (BRENDA, IUBMB)
Numéro CE :
232-656-0
Numéro MDL:
MFCD00070858
Code UNSPSC :
12352204
eCl@ss :
32160410
Nomenclature NACRES :
NA.54

Type

Type IX

Forme

powder

Activité spécifique

50,000-100,000 units/g solid

Poids mol.

~544620 Da

Température de stockage

2-8°C

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Description générale

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease (Ure) enzyme is present in the cytosol. It is active at acidic pH and is expressed at 28°C. Urease binds to nickel and is present in archaea, bacteria, unicellular eukaryotes and plants. Jack bean urease was the first enzyme to be crystallized and the first enzyme found to contain nickel. It is a multi-subunit enzyme, consisting of 91 kDa subunits in three protein forms.

Application

Urease from Canavalia ensiformis (Jack bean) has been used: to spike urine samples to determine the hydrolysis rate of urea in pure urine It has also been used to study the inhibitory action of polyhalogenated benzo- and naphthoquinones on Bacillus pasteurii and Canavalia ensiformis.

Actions biochimiques/physiologiques

In Canavalia ensiformis (Jack Bean), urease facilitates the conversion of L-canavanine to canaline.
Urease is involved in purine metabolism and the urea cycle. It hydrolyze urea to produce ammonia and carbon dioxide.

Définition de l'unité

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Pictogrammes

Health hazardExclamation mark
GHS08,GHS07

Mention d'avertissement

Danger

Mentions de danger

H315,H319,H334,H335

Classification des risques

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Organes cibles

Respiratory system

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

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Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform
Kunduru KR, et al.
Test, 590, 143-167 (2017)
G A Rosenthal et al.
Plant physiology, 76(2), 541-544 (1984-10-01)
l-Canavanine, the guanidinooxy structural analog of l-arginine, is an important nonprotein amino acid of many leguminous plants with nitrogen storage a major proported role. l-[Guanidinooxy-(14)C]canavanine, [(14)C] urea, and [(15)N]urea were injected separately into the fleshy, green cotyledons of 9-day old
An investigation of the use of urease-antibody conjugates in enzyme immunoassays
Chandler HM, et al.
Journal of Immunological Methods, 53(2), 187-194 (1982)
Xavier Arqué et al.
ACS nano, 16(5), 7547-7558 (2022-04-30)
The increasing resistance of bacteria to existing antibiotics constitutes a major public health threat globally. Most current antibiotic treatments are hindered by poor delivery to the infection site, leading to undesired off-target effects and drug resistance development and spread. Here
Morgane Valles et al.
ACS nano (2022-03-29)
Enzyme-powered micro- and nanomotors make use of biocatalysis to self-propel in aqueous media and hold immense promise for active and targeted drug delivery. Most (if not all) of these micro- and nanomotors described to date are fabricated using a commercially
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