SRP3044

Flt-3 Ligand human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonyme(s) : Fms-related tyrosine kinase 3 ligand
• Code UNSPSC : 12352200
• Nomenclature NACRES : NA.32

Propriétés

• Source biologique: human
• Produit recombinant: expressed in E. coli
• Pureté: ≥98% (HPLC); ≥98% (SDS-PAGE)
• Forme: lyophilized
• Puissance: ≤10 ng/mL ED₅₀ ((OCI-AML5 cell proliferation))
• Poids mol.: 17.6 kDa
• Conditionnement: pkg of 10 μg
• Technique(s): cell culture | mammalian: suitable
• Impuretés: <0.1 EU/μg endotoxin, tested
• Couleur: white to off-white
• Numéro d'accès UniProt: P49771
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Informations sur le gène: human ... FLT3LG(2323)

Description

Description générale

FLT3LG (Fms-related tyrosine kinase 3 ligand) is a transmembrane glycoprotein and a ligand for FLT3 receptor. It is widely expressed in human tissues. The protein exists in membrane bound as well as secreted forms. Binding of FLT3LG to FLT3 results in receptor dimerization, activation of the tyrosine kinase domain, autophosphorylation and thereby activation of the receptor. Recombinant human Flt3-Ligand is a soluble 17.6kDa protein consisting of 155 amino acid residues.

Actions biochimiques/physiologiques

FLT3LG (Fms-related tyrosine kinase 3 ligand) is a hematopoietic growth factor. It plays a significant role in the development of myeloid and plasmacytoid dendritic cells. It is also involved in the expansion of Treg (T regulatory) cells, thereby offering treatment for autoimmune disorders and transplantation processes. FLT3LG is associated with autoimmune arthritis, for instance rheumatoid arthritis and primary Sjogren′s syndrome. FLT3LG along with FLT3 is involved in the proliferation of primary AML (acute myeloid leukemia) blasts and autocrine signaling in AML.

Séquence

MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT

Forme physique

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM NaCl, pH 7.5

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable