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SAB5600096

Sigma-Aldrich

Anti-His-Tag Chimeric antibody, Human Monoclonal

recombinant, expressed in HEK 293 cells, clone RMH01, purified immunoglobulin

Synonyme(s) :

Anti-His tag antibody

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Produit recombinant

expressed in HEK 293 cells

Niveau de qualité

100

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

RMH01, monoclonal
recombinant monoclonal

Forme

buffered aqueous glycerol solution

Technique(s)

capture ELISA: 0.1-2 μg/mL
western blot: 0.1-1 μg/mL

Isotype

IgG

Conditions d'expédition

wet ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Description générale

Hexahistidine tag (His-tag) consists of four to six histidines fused genetically. It is widely used to purify proteins by immobilized metal-ion affinity chromatography (IMAC).

Spécificité

This antibody is a Human-Rabbit chimeric antibody, made by fusion of the antigen binding region (variable domains of the heavy and light chains, VH and VL) of rabbit anti-HisTag monoclonal antibody (RM146), with the constant domain of Human IgG1. The antibody is recognized only by an anti-Human IgG secondary antibody.

Immunogène

Mixture of a peptide with 6xHis-Tag at the N-terminus and another peptide with 6xHis-Tag at the C-terminus.

Caractéristiques et avantages

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Forme physique

Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Jonathan F Lovell et al.
BMC medicine, 20(1), 462-462 (2022-11-30)
Numerous vaccine strategies are being advanced to control SARS-CoV-2, the cause of the COVID-19 pandemic. EuCorVac-19 (ECV19) is a recombinant protein nanoparticle vaccine that displays the SARS-CoV-2 receptor-binding domain (RBD) on immunogenic nanoliposomes. Initial study of a phase 2 randomized
Tandem immobilized metal-ion affinity chromatography/immunoaffinity purification of His-tagged proteins?evaluation of two anti-His-tag monoclonal antibodies
Muller KM, et al.
Analytical Biochemistry, 259(1), 54-61 (1998)
Loree Heller et al.
Journal of cellular and molecular medicine, 24(15), 8772-8778 (2020-07-02)
In neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, multiple sclerosis and amyotrophic lateral sclerosis, neuroinflammation can lead to blood-brain barrier (BBB) breakdown. After intravenous or intra-arterial injection into mice, endothelial progenitor cells (EPCs) home to the damaged BBB to
Reynald Thinard et al.
Pharmaceutics, 14(7) (2022-07-28)
The failures of anti-β-amyloid immunotherapies suggested that the very low fraction of injected antibodies reaching the brain parenchyma due to the filtering effect of the BBB may be a reason for the lack of therapeutic effect. However, there is no

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