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Key Documents

P6246

Sigma-Aldrich

Anti-PABP antibody, Mouse monoclonal

clone 10E10, purified from hybridoma cell culture

Synonyme(s) :

Anti-Poly(A)-Binding Protein

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

unconjugated

Forme d'anticorps

purified from hybridoma cell culture

Type de produit anticorps

primary antibodies

Clone

10E10, monoclonal

Forme

buffered aqueous solution

Poids mol.

antigen ~69 kDa

Espèces réactives

hamster, canine, bovine, Xenopus, human, monkey

Technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using whole cell extracts of HEK 293T cells

Isotype

IgG1

Numéro d'accès UniProt

Conditions d'expédition

dry ice

Température de stockage

−20°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... PABPC1(26986)

Description générale

Monoclonal Anti-PABP (mouse IgG1 isotype) is derived from the 10E10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from BALB/c mice immunized with recombinant human PABP. Poly(A) binding protein (PABP) is highly conserved between different organisms especially at the amino-terminal region of the protein that contains four RNA-binding domains (RBDs).
Poly(A) binding protein (PABP) possesses RNA recognition motifs (RRMs), a proline-rich region and a PABP C-terminal domain. The gene encoding this protein is localized on human chromosome 8q22.3.

Immunogène

recombinant human PABP.

Application

Monoclonal Anti-PABP antibody produced in mouse has been used for immunofluorescence.
Monoclonal Anti-PABP antibody produced in mouse has been used in:
  • Immunoblotting
  • Immunostaining
  • Immunoprecipitation
  • Enzyme linked immunosorbent assay(ELISA)
  • Immunocytochemistry

Actions biochimiques/physiologiques

Poly(A) binding protein (PABP) has a role in mRNA translation and stability. It modulates gene expression. The protein binds to the poly(A) tail, bridges the ends of mRNA and forms a closed loop. This might help in translation initiation by promoting ribosome recruitment.
Poly(A) binding protein (PABP) is found in excess, relative to the amount of cytoplasmic poly(A) (three fold) and binds to its poly(A) target at high affinity (Kd of 7nM). The eukaryotic initiation factor, EIF4F complex, interacts with PABP indirectly via the PAIP-1 protein (PABP-interacting protein-1). As a consequence, an interaction between the 5′ and 3′ ends of mRNA occurs. This proximity between the mRNA ends contributes to its stability and enhances translation, since terminating ribosomes may start translation again.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

RNA UK 2012: The role of mammalian poly(A)-binding proteins in co-ordinating mRNA turnover
Brook M and Gray NK
Biochemical Society Transactions, 40(Pt 4), 856-856 (2012)
An integrative approach to identify YB-1-interacting proteins required for cisplatin resistance in MCF7 and MDA-MB-231 breast cancer cells
Chantal Garand
Cancer Science (2011)
BTG2 bridges PABPC1 RNA-binding domains and CAF1 deadenylase to control cell proliferation
Stupfler B, et al.
Nature Communications, 7, 10811-10811 (2016)
Poly (A)-binding proteins: multifunctional scaffolds for the post-transcriptional control of gene expression
Brook M and Gray NK
Genome Biology, 4(7), 223-223 (2003)
Hun-Way Hwang et al.
Cell reports, 15(2), 423-435 (2016-04-07)
Accurate and precise annotation of 3' UTRs is critical for understanding how mRNAs are regulated by microRNAs (miRNAs) and RNA-binding proteins (RBPs). Here, we describe a method, poly(A) binding protein-mediated mRNA 3' end retrieval by crosslinking immunoprecipitation (PAPERCLIP), that shows

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