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P4978

Sigma-Aldrich

Phosphatase, Alkaline from calf intestine

buffered aqueous glycerol solution

Synonyme(s) :

CIAP, CIP, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)

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About This Item

Numéro CAS:
9001-78-9
Numéro de classification (Commission des enzymes):
3.1.3.1 (BRENDA, IUBMB)
Numéro MDL:
MFCD00131849
Code UNSPSC :
12352204
eCl@ss :
42010105
Nomenclature NACRES :
NA.53

Qualité

for molecular biology

Forme

buffered aqueous glycerol solution

Poids mol.

~80 kDa

Concentration

≥10,000 units/mL

Numéro d'accès UniProt

P19111

Activité étrangère

DNase, RNase, none detected

Conditions d'expédition

wet ice

Température de stockage

−20°C

Informations sur le gène

cow ... ALPI(280993)

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Description générale

Alkaline phosphatase is a phosphomonoesterase that catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.

Application

Commonly used to remove the 5′-terminal phosphate from nucleic acids during molecular cloning reactions.

Composants

Alkaline phosphatase is provided in a solution of 10 mM Tris-HCl (pH 8.2), 50 mM KCl, 1 mM MgCl2, and 0.1 mM ZnCl2, in 50% (w/v) glycerol. 10X CIP Buffer, Product Number C3225, is included.

Définition de l'unité

One unit will hydrolyze 1 μmole of p-nitrophenyl phosphate per min at 37 °C.

Autres remarques

Alkaline phosphatase in 5 mM EDTA (pH 8) will be irreversibly heat inactivated at 75 °C for 10 minutes.

Inhibiteur

Réf. du produit
Description
Tarif

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

D9380

DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution

R0884

T7 RNA Polymerase, recombinant, expressed in E. coli, buffered aqueous solution

D8276

DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution

D2886

DNA Ligase from T4-infected Escherichia coli, buffered aqueous glycerol solution

P4390

Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution

G5663

Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

R6513

Ribonucléase A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R4642

Ribonucléase A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

P6911

Protéase from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG1

DNA Ligation Kit

Substrat

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Christopher J Moore et al.
Genes & development, 35(3-4), 286-299 (2021-01-16)
RNase E is an essential, multifunctional ribonuclease encoded in E. coli by the rne gene. Structural analysis indicates that the ribonucleolytic activity of this enzyme is conferred by rne-encoded polypeptide chains that (1) dimerize to form a catalytic site at
Yusheng Zhao et al.
Genes & development, 35(11-12), 888-898 (2021-05-15)
Plants monitor many aspects of their fluctuating environments to help align their development with seasons. Molecular understanding of how noisy temperature cues are registered has emerged from dissection of vernalization in Arabidopsis, which involves a multiphase cold-dependent silencing of the
J Juhásová et al.
Physiological research, 60(3), 559-571 (2011-03-16)
Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs
A D Bakker et al.
Journal of dental research, 93(4), 394-399 (2014-02-05)
Mechanosensitive osteocytes regulate bone mass in adults. Interleukin 6 (IL-6), such as present during orthodontic tooth movement, also strongly affects bone mass, but little is known about the effect of IL-6 on osteocyte function. Therefore we aimed to determine in
Yixing Cheng et al.
Journal of biomedical nanotechnology, 10(2), 287-298 (2014-04-18)
Scaffold architecture, surface topography, biochemical and mechanical cues have been shown to significantly improve cellular events and in vivo tissue regeneration. Specifically electrospun nanofiber matrices have gained tremendous interest due to their intrinsic structural resemblance to native tissue extracellular matrix
Afficher tous les articles scientifiques apparentés

Protocoles

Calf Intestinal (CIP) Alkaline Phosphatase for Nucleic Acid Dephosphorylation

CIP is used to remove 5’-phosphate groups from DNA, RNA and both ribo and deoxy-ribonucleoside triphosphates. Detailed protocol on how to dephosphorylate DNA.

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

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