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P2983

Millipore

Anticorps monoclonal ANTI-FLAG® M2 antibody produced in mouse

96-well, clear, polystyrene, flat bottom plate

Synonyme(s) :

Anti-ddddk, Anti-dykddddk

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About This Item

Code UNSPSC :
12352203
Nomenclature NACRES :
NA.32

Type de produit anticorps

primary antibodies

Niveau de qualité

200

Matériaux

polystyrene

Clone

monoclonal

Durée de conservation

Unopened plates are stable for 2 years. Once opened they are stable for 2 weeks.

Technique(s)

ELISA: suitable

Isotype

IgG1

Capacité

100-300 ng/well

Température de stockage

2-8°C

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Catégories apparentées

Description générale

The Anti-FLAG® HS, M2 Coated Plates are designed to ensure a higher binding capacity than other plates. This is due to the orientation of the Anti-FLAG® M2 mouse monoclonal IgG1 antibody on the plates. The antibody is covalently linked to the surface of a microtiter plate via the Fc portion of the antibody.

Application

ANTI-FLAG® High Sensitivity, M2 coated 96-well plates is used for Enzyme-linked immunosorbent assays. A convenient, ready to use, platform for the capture and detection of FLAG® fusion proteins. The ANTI-FLAG® M2 antibody is covalently attached to the surface through the Fc portion and can detect 1 ng FLAG fusion protein/well with a capacity of up to 300ng/well. Suitable for screening for expression, study of protein:protein interactions and ELISA assays. Manufactured under ISO 9002 in Sigma′s GMP facility, ANTI-FLAG®P2983 high sensitivity M2 coated multiwell plates utilize a flat bottom, polystyrene baseplate.

Learn more product details in our FLAG® application portal.

Stockage et stabilité

Once plates are opened, they should be stored with desiccant at 2- 8 °C and used within two weeks.

Autres remarques

Components:
The plate is supplied as a 96-well microtiter plate with clear sides and bottom.
Coating:
ANTI-FLAG® M2 mouse monoclonal antibody, IgG1, is coated at a reaction volume of 200 ml/well.
Blocking:
The wells are pre-blocked for convenience at 275 to 300 ml/well with a complex solution containing bovine serum albumin.
Specificity:
The plates are specific for the FLAG epitope regardless of its placement in the fusion protein: amino-terminal, Met-amino terminal, carboxy terminal or internal. Binding of the epitope is not Ca2+ dependent.
Sensitivity:
Detection of 1 ng/well of a control fusion protein was observed in an ELISA format with p-Nitrophenyl Phosphate (pNPP) as a substrate.
Capacity:
Capture of 100 to 300 ng/well of a FLAG fusion protein has been demonstrated.

Informations légales

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
F-127 is a registered trademark of BASF SE
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Description
Tarif

08168

Timestrip Plus™ 8 °C

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Natalie F Shanks et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 34(36), 12104-12120 (2014-09-05)
Cornichon homologs (CNIHs) are AMPA-type glutamate receptor (AMPAR) auxiliary subunits that modulate AMPAR ion channel function and trafficking. Mechanisms underlying this interaction and functional modulation of the receptor complex are currently unclear. Here, using proteins expressed from mouse and rat
Molecular dissection of the interaction between the AMPA receptor and cornichon homolog-3.
Shanks NF, Cais O, Maruo T, et al.
The Journal of Neuroscience, 34(36), 12104-12120 (2014)
Shu Kachi et al.
Human gene therapy, 20(1), 31-39 (2009-01-01)
Equine infectious anemia virus (EIAV) is a nonprimate lentivirus that does not cause human disease. Subretinal injection into mice of a recombinant EIAV lentiviral vector in which lacZ is driven by a CMV promoter (EIAV CMV LacZ) resulted in rapid
Qiongming Liu et al.
BMC biotechnology, 10, 78-78 (2010-10-29)
Analysis of protein-protein interactions (PPIs) is a valuable approach for the characterization of huge networks of protein complexes or proteins of unknown function. Co-immunoprecipitation (coIP) using affinity resins coupled to protein A/G is the most widely used method for PPI
FLAG 96-well Immunoprecipitation System for High-Throughput Protein-Protein Interaction Studies
Uder, S., et al.
Molecular Biology, 4 null
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