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MAK443

Sigma-Aldrich

L-Lactate Assay Kit

Sufficient for 100 fluorometric tests

Synonyme(s) :

ʟ-Lactic Acid Assay Kit

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About This Item

Code UNSPSC :
12161503
Nomenclature NACRES :
NA.84

Niveau de qualité

100

Entrée

cell culture medium
plasma
serum
urine

Méthode de détection

fluorometric

Maladie(s) pertinente(s)

diabetes; pulmonary disorders; endocrinological disorders; cardiovascular diseases; cancer

Température de stockage

−20°C

Description générale

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.

Application

The L-Lactate Assay Kit may be used for:
  • Lactate Metabolism Research
  • Tissue Metabolism Research
  • Drug Discovery

Caractéristiques et avantages

  • Simplified Process: Experience a streamlined process with the addition of only a single working reagent, reducing complexity and saving valuable time and effort.
  • Broader Detection Range: Detect L-Lactate levels accurately across a wide range, from 1 to 50 μM, using just 50 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.
  • High-Throughput Compatibility: Effortlessly integrate our kit into high-throughput handling systems, ensuring seamless operation and maintaining efficiency and accuracy in large-scale analyses.

Adéquation

The kit is suitable for the quantitative determination of L-lactate (L-lactic acid) and the evaluation of drug effects on its metabolism in serum, plasma, urine, cell culture media, and other biological samples.

Principe

The L-Lactate Assay Kit is based on the lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λEx = 530 nm/λEm = 585 nm, is proportional to the lactate concentration in the sample.

Autres remarques

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Produit(s) apparenté(s)

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Description
Tarif

Code de la classe de stockage

12 - Non Combustible Liquids

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