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Key Documents

L8262

Sigma-Aldrich

Lectin from Ulex europaeus (gorse, furze)

biotin conjugate, lyophilized powder

Synonyme(s) :

Ulex europaeus agglutinin, UEA-I

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Conjugué

biotin conjugate

Niveau de qualité

200

Forme

lyophilized powder

Puissance

<50 μg/mL agglutination activity

Composition

Protein, ~85% E1%/280

Ampleur du marquage

~3 mol biotin per mol protein

Température de stockage

2-8°C

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Application

Lectin from Ulex europaeus (gorse, furze) has been used:
  • as a Paneth cells marker in immunohistochemistry at 1:1,000 dilution
  • in double staining for endothelial cells for quantitation of blood vessels
  • in histochemical staining of bronchial epithelial cells

Actions biochimiques/physiologiques

Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.

Conditionnement

Package size based on protein content.

Liaison

Conjugates are prepared from affinity purified lectin.

Forme physique

Contains sodium citrate

Remarque sur l'analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

M Tango et al.
Archives of histology and cytology, 63(1), 81-89 (2000-04-19)
The aggregation of lymphoid cells in the bronchial mucosa has been named the bronchus-associated lymphoid tissue (BALT) and investigated in comparison with the gut-associated lymphoid tissue (GALT). To elucidate precisely the structure and function of the BALT, the present study
Maria Hernandez et al.
Biomedicines, 9(11) (2021-11-28)
The purpose of this study was to assess vascular and histological alterations in two COVID-19 and three control post-mortem retinas. The macular areas of flat-mounted samples were processed for immunofluorescence. Lectin and collagen IV positive vessels were captured under confocal
Daisuke Kondoh et al.
Cells, tissues, organs, 203(1), 20-28 (2016-07-18)
Glycoconjugates in the olfactory system play critical roles in neuronal formation, and α1-2 fucose (α1-2Fuc) glycan mediates neurite outgrowth and synaptic plasticity. Histochemical findings of α1-2Fuc glycan in the mouse olfactory system detected using Ulex europaeus agglutinin-I (UEA-I) vary. This
Sarah Waters et al.
Acta neuropathologica communications, 9(1), 144-144 (2021-08-28)
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disease involving progressive degeneration of upper and lower motor neurons. The pattern of lower motor neuron loss along the spinal cord follows the pattern of deposition of phosphorylated TDP-43 aggregates. The blood-spinal
Heather Ireland et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 233(4), 1332-1336 (2005-06-07)
Here, we exploit an absolute differential between stem and progeny cells in their ability to express Cre from a somatically inducible transgene to determine the longevity of intestinal Paneth cells. In the Ahcre transgenic line induction of Cre recombinase allows
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