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H6666

Sigma-Aldrich

Facteur de croissance des kératinocytes human

HumanKine®, >95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

Synonyme(s) :

FGF-7, KGF

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About This Item

Numéro CAS:
148348-15-6
Numéro MDL:
MFCD00212245
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

product name

Facteur de croissance des kératinocytes human, KGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Source biologique

human

Produit recombinant

expressed in HEK 293 cells

Pureté

>95% (SDS-PAGE)

Forme

lyophilized powder

Puissance

4-20 ng/mL EC50

Qualité

endotoxin tested

Poids mol.

dimer 17-30 kDa (glycosylated)

Conditionnement

pkg of 10 μg

Technique(s)

cell culture | mammalian: suitable

Impuretés

<1 EU/μg

Numéro d'accès UniProt

P21781

Température de stockage

−20°C

Informations sur le gène

human ... FGF7(2252)

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Catégories apparentées

Description générale

Keratinocyte Growth Factor (KGF) belongs to the fibroblast growth factor 7 (FGF-7) family. It is produced by the mesenchymal cells, γδ T cells, and fibroblasts in several organs. The KGF gene is located on the human chromosome at 15q21.2.

Application

Keratinocyte Growth Factor (KGF) human has been used as a component of the keratinocyte differentiation medium.

Actions biochimiques/physiologiques

Keratinocyte Growth Factor (KGF) exerts paracrine effects on mesenchymal cells. It exhibits cryoprotective effects on epithelial cells of the lungs, gastrointestinal tract, and the lungs. KGF participates in the activation of IIIb isoform of the fibroblast growth factor (FGF)-receptor 2 (FGFR2-IIIb) present on keratinocytes. This activation further favors proliferation, migration, and survival of keratinocytes.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Notes préparatoires

HumanKine Keratinocyte Growth Factor (KGF) is expressed as glycosylated 17 and 30 kDa monomers in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Remarque sur l'analyse

The activity was determined by the dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

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Description
Tarif

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

Michael J LaFemina et al.
American journal of physiology. Lung cellular and molecular physiology, 299(6), L724-L734 (2010-09-14)
Keratinocyte growth factor (KGF) has efficacy in several experimental models of lung injury; however, the mechanisms underlying KGF's protective effect remain incompletely understood. This study was undertaken to determine whether KGF augments barrier function in primary rat alveolar epithelial cells
Qandeel Fatima et al.
Open life sciences, 13, 544-552 (2018-12-31)
Autologous skin grafts are used to treat severe burn wounds, however, the availability of adequate donor sites makes this option less practical. Recently, stem cells have been used successfully in tissue engineering and in regenerative medicine. The current study aims
Umbilical cord tissue derived mesenchymal stem cells can differentiate into skin cells
Fatima Q, et al.
The Open Immunology Journal, 13(1), 544-552 (2018)
Susanne Braun et al.
Journal of cell science, 119(Pt 23), 4841-4849 (2006-11-09)
Owing to its potent cytoprotective properties for epithelial cells, keratinocyte growth factor (KGF) is successfully used for the treatment of chemotherapy- and radiotherapy-induced oral mucositis in cancer patients. It is therefore of major interest to determine possible clinical applications of
Verônica Chomiski et al.
Acta cirurgica brasileira, 31(8), 505-512 (2016-09-01)
To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients)
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