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Key Documents

GE28-9180-08

PD Miditrap G-25

Cytiva 28-9180-08

Synonyme(s) :

G-25 Miditrap, Miditrap G-25, PD G-25 Miditrap

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About This Item

Code UNSPSC :
41115709
Nomenclature NACRES :
NA.56

Caractéristiques

wetted part: no

Conditionnement

pkg of 50 ea (columns)

Fabricant/nom de marque

Cytiva 28-9180-08

Conditions de stockage

dry media

Taille du lit

13 mm × 26 mm

Volume du lit

3.5 mL

D.I. de la colonne

13 mm

Matrice

cross-linked dextran

Taille des particules

>50 μm (dry)
85-260 μm

cleaning

2-13

working range

2-13

Adéquation

suitable for bioprocess medium

Catégories apparentées

Description générale

PD MidiTrap G-25 is a convenient and rapid cleanup with high reproducibility and the potential for high throughput by running multiple samples in parallel. Can be used in a centrifugation or gravity protocol.

PD MidiTrap G-25 is designed for convenient desalting and buffer exchange of biological samples and volumes up to 1.0 mL can be handled. Two alternative application protocols are available, gravity or centrifugation. By using the gravity protocol, a simple cleanup of the sample is done with one or several columns in parallel without any need for a purification system. With the centrifugation protocol, the samples are run in parallel in a standard centrifuge with minimal dilution of the eluted sample. Four adapters are included in each product pack to enable easy centrifugation.

Application

Protein sample preparation. Desalting/Buffer Exchange/Cleanup.

Caractéristiques et avantages

  • Reliable and reproducible cleanup of proteins and other large biomolecules (>5000 Mr).
  • Range of applications such as desalting, buffer exchange, and removal of low-molecular weight compounds.
  • High desalting capacity.
  • Flexible protocols with ability to use gravity flow or centrifugation.
  • Sample preparation in the range of 0.5 to 1.0 mL for gravity and 0.75 to 1.0 mL with centrifugation.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (0.15% Kathon CG/ICP Biocide)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H317

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 2

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Articles

Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns

This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocoles

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

Western Blotting Sample Preparation

Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.

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