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GE17-1279-02

rProtein A Sepharose™ Fast Flow

Cytiva 17-1279-02, pack of 25 mL

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About This Item

Code UNSPSC :
41106500
Nomenclature NACRES :
NA.56

ligand

recombinant protein A (E. coli)

Conditionnement

pack of 25 mL

Fabricant/nom de marque

Cytiva 17-1279-02

Conditions de stockage

(20% Ehtanol)

Matrice

4% cross-linked agarose

Taille des particules

60-165 μm

Diamètre moyen

90 μm (d50v)

cleaning in place

2-11

working range

3-10

Capacité

~50 mg binding capacity (human lgG/ml medium)

Adéquation

suitable for bioprocess medium

Température de stockage

2-8°C

Description générale

rProtein A Sepharose 4 Fast Flow is an affinity resin for antibody purification at laboratory and process scale.

Monoclonal and polyclonal antibody purification

rProtein A Sepharose 4 Fast Flow is built upon the well-established Sepharose Fast Flow platform and is used in many approved monoclonal antibody processes. It is composed of recombinant protein A coupled to the cross-linked 4% agarose base matrix.The highly crosslinked agarose resin enables high flow rates and high productivity.

Protein A resin characteristics

The recombinant protein is produced in E. coli and engineered with an oriented coupling to enhance binding capacity. Epoxy-based coupling ensures low ligand leakage. Specificity of the recombinant protein for the Fc region of IgG is similar to the native protein, giving a high degree of selectivity and yield.

Streamlined scale up

Scale-up with this resin is straightforward and is further aided by a range of prepacked process development tools, making purification scale-up straightforward.

Caractéristiques et avantages

  • For monoclonal and polyclonal antibody purification at both laboratory and process scale.
  • Well established among Protein A resins as excellent for monoclonal antibody (mAb) processes.
  • Free from animal-derived components.
  • Hydrophilic base matrix ensures low levels of non-specific binding and low levels of host cell-derived impurities in the elution pool.
  • Fulfills industrial demands for security of supply, robust performance, and regulatory support.

Stockage et stabilité

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Remarque sur l'analyse

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informations légales

Sepharose is a trademark of Cytiva

Pictogrammes

Flame
GHS02

Mention d'avertissement

Warning

Mentions de danger

H226

Code de la classe de stockage

3 - Flammable liquids

Certificats d'analyse (COA)

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Protocoles

Column Cleaning for Ion Exchange Chromatography and Chromatofocusing

This page covers detailed information on cleaning procedures and recommended flow for column cleaning.

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