Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

D2886

Sigma-Aldrich

DNA Ligase from T4-infected Escherichia coli

buffered aqueous glycerol solution

Synonyme(s) :

Polydeoxyribonucleotide Synthase, Polynucleotide Ligase, T4 DNA Ligase

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Numéro CAS:
9015-85-4
Numéro de classification (Commission des enzymes):
6.5.1.1 (BRENDA, IUBMB)
Numéro CE :
232-770-0
Numéro MDL:
MFCD00163508
Code UNSPSC :
12352204
Nomenclature NACRES :
NA.53

Qualité

for molecular biology

Forme

buffered aqueous glycerol solution

Activité spécifique

4,000 U/mL

Poids mol.

68 kDa

Numéro d'accès UniProt

P00970

Température de stockage

−20°C

Informations sur le gène

bacteriophage T4 ... 30(1258680)

Vous recherchez des produits similaires ? Visite Guide de comparaison des produits

Catégories apparentées

Application

Suitable for:
  • Ligation of blunt ended or cohesive DNA fragments
  • Ligation of cloning vector and restriction insert fragments
  • Seal nicks in double stranded DNA and RNA or DNA/RNA hybrids
  • Couple RNA single strands by bridging oligonucleotide adapters

Actions biochimiques/physiologiques

T4 DNA Ligase forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. The ligation reaction requires ATP as a cofactor. Ligation of blunt-ended fragments requires higher enzyme concentration and can be facilitated by using PEG in the reaction mixture. The enzyme requires a 3′ hydroxyl and 5′ phosphate for ligation. Self-ligation of vector DNA can be prevented by dephosphorylation with alkaline phosphatase. T4 ligase plays an active role in repair of DNA and RNA nicks.

Composants

T4 DNA Ligase is supplied in a solution containing 20 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, and 50% (v/v) glycerol.

Définition de l'unité

One Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of P32 from pyrophosphate into ATP as Norit-absorbable material in 20 minutes at 37°C.

Autres remarques

T4 DNA Ligase is inactivated by heating at 65 °C for 10 minutes.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

D9380

DNA Polymerase I from Escherichia coli lysogenic for NM 964, buffered aqueous glycerol solution

R0884

T7 RNA Polymerase, recombinant, expressed in E. coli, buffered aqueous solution

D8276

DNA Polymerase I, Klenow Fragment from Escherichia coli, buffered aqueous glycerol solution

P4978

Phosphatase, Alkaline from calf intestine, buffered aqueous glycerol solution

P4390

Polynucleotide Kinase from T4-infected Escherichia coli, 10 units/μL, buffered aqueous glycerol solution

G5663

Glutathione S-Transferase from E. coli, recombinant, expressed in E. coli, buffered aqueous solution

D7291

Deoxyribonuclease I RNase-free solution from bovine pancreas

R6513

Ribonucléase A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

R4642

Ribonucléase A from bovine pancreas, (Solution of 50% glycerol, 10mM Tris-HCL pH 8.0)

P6911

Protéase from Streptomyces griseus, BioReagent, DNase, RNase, and nickase, none detected (No RNase.)

LIG2

QuickLink™ DNA Ligation Kit, joins blunt-end and sticky-end DNA fragments

LIG1

DNA Ligation Kit

Pictogrammes

Health hazard
GHS08

Mention d'avertissement

Danger

Mentions de danger

H334

Conseils de prudence

P261 - P284 - P501

Classification des risques

Resp. Sens. 1

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

M J Moore et al.
Science (New York, N.Y.), 256(5059), 992-997 (1992-05-15)
A simple and efficient method for synthesizing long, site-specifically modified RNA molecules was developed whereby segments of RNA were joined with the use of bacteriophage T4 DNA ligase. A single hydrogen or O-methyl group was substituted for the 2'-hydroxyl group
Hiroshi Ochiai et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(27), 10915-10920 (2012-06-20)
To understand complex biological systems, such as the development of multicellular organisms, it is important to characterize the gene expression dynamics. However, there is currently no universal technique for targeted insertion of reporter genes and quantitative imaging in multicellular model
Engler, M.J. and Richardson, C.C. et al.
The Enzymes, 5, 3-3 (1982)
Xin Cheng et al.
European journal of cell biology, 91(10), 782-788 (2012-08-04)
Translocation of mitochondrial DNA (mtDNA) fragments to the nucleus and insertion of those fragments into nuclear DNA has been observed in several organisms ranging from yeast to plants and mammals. Disruption of specific nuclear genes by de novo insertions of
Wenxin Gu et al.
Bioorganic & medicinal chemistry letters, 22(11), 3693-3698 (2012-05-09)
A series of 2,6-disubstituted aminoalkoxypyrimidine carboxamides (AAPCs) with potent inhibition of bacterial NAD(+)-dependent DNA ligase was discovered through the use of structure-guided design. Two subsites in the NAD(+)-binding pocket were explored to modulate enzyme inhibitory potency: a hydrophobic selectivity region
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique