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Key Documents

CS0030

Sigma-Aldrich

Senescence Cells Histochemical Staining Kit

sufficient for 100 tests

Synonyme(s) :

Senescent cells IHC kit, cellular β-galactosidase probe

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About This Item

Code UNSPSC :
12352207
Nomenclature NACRES :
NA.32

Utilisation

sufficient for 100 tests

Niveau de qualité

Conditionnement

pkg of 1 kit

Conditions de stockage

dry at room temperature

Méthode de détection

colorimetric

Conditions d'expédition

dry ice

Température de stockage

−20°C

Description générale

The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for ß-galactosidase activity at pH 6. ß-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells.
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time.

Application

Senescence Cells Histochemical Staining Kit has been used for:
  • senescence-associated β-galactosidase assay
  • to test for senescence in human pancreatic cancer cells
  • adipose-derived stem cells (ADSCs)
  • cord blood mesenchymal stromal/stem cells (CBMSC)

Actions biochimiques/physiologiques

Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.

Informations légales

Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.

Composants de kit seuls

Réf. du produit
Description

  • X-gal solution 4 mL

  • Staining Solution, 10X 15 mL

  • Fixation Buffer 10x 15 mL

  • Reagent B 1.5 mL

  • Reagent C 1.5 mL

  • Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Pictogrammes

Skull and crossbonesHealth hazardCorrosion

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Organes cibles

Respiratory system

Risques supp

Code de la classe de stockage

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificats d'analyse (COA)

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