TF1 Cell Line human

93022307, human blood, Not specified

• Synonyme(s) : Human TF1 Cells, TF1 Cell Culture
• Code UNSPSC : 41106514

Propriétés

• product name: TF1 Cell Line human, 93022307, Erythroleukemic cell line from blood.
• Source biologique: human blood
• Mode de croissance: Suspension
• Caryotype: Carries homogeneous chromosomal abnormality (54,X)
• Morphologie: Not specified
• Produits: Not specified
• Récepteurs: Interleukin 3 (IL-3) and Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
• Technique(s): cell culture | mammalian: suitable
• Maladie(s) pertinente(s): cancer
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human erythroleukaemia

Description de la lignée cellulaire

TF-1 have been derived from a patient with erythroleukaemia. They show complete growth dependency on GM-CSF or IL-3, and carry a homogeneous chromosomal abnormality (54,X). Constitutive expression of globin genes indicate commitment of TF-1 to the erythroid lineage, although glycophorin A or carbonyl anhydrase I are not expressed. On incubation with hemin or delta-aminolevulinic acid haemoglobin synthesis was induced, whereas TPA induced differentiation into macrophage-like cells. Erythroprotein also sustains short-term growth, but does not induce differentiation.

Application

TF1 cell line has been used-

• for tumorigenic studies
• to study the effect of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) on proliferation and TNFR2 expression in TF1 cells
• in a cell-based proliferation assay to study the biological activities of anti-IL-6R Nanobody ALX-0061
• to study the induction of STAT5 phosphorylation by IL-3

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
THO1: 7,9
TPOX: 8
vWA: 15,17

Milieu de culture

RPMI 1640 + 2mM Glutamine + 1% Sodium Pyruvate (NaP) + 2-5ng/ml Human Granulocyte Macrophage Colony Stimulating Factor (Human GM-CSF) + 10% Foetal Bovine Serum (FBS). It is recommended to add fresh GM-CSF every 48 hours.

Procédure de repiquage

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C.

Autres remarques

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