LL24

90073001, human lung, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: LL24, 90073001
• Source biologique: human lung
• Mode de croissance: Adherent
• Caryotype: 2n = 46; diploid
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human diploid lung

Description de la lignée cellulaire

Derived from normal tissue taken at post-mortem from the lung of a 5 year old male. An ampoule of cells frozen after one passage and estimated population doubling (PDL) 2 was submitted to the American Type Culture Collection in June, 1977. Derived populations at passage 6 and approximately 14 PDL are available for distribution. Progeny were found to undergo an additional 25 population doublings in longevity trials.

Application

Tumourigenicity studies

Milieu de culture

Ham′s F12 + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, a further 20 population doublings expected.

Autres remarques

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