153BR

90011808, human skin, Fibroblast

• Code UNSPSC : 41106514

Propriétés

• product name: 153BR, 90011808
• Source biologique: human skin
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Fibroblast
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Human skin fibroblast

Description de la lignée cellulaire

Established from a biopsy of a normal 56 year old male. The cells have been used in radiation survival studies. EBV-transformed lymphocytes designated LB185 (Sigma Catalogue number. 98062318) have been isolated from the same individual. This has been confirmed by STR profiling at ECACC.

Application

DNA repair studies

Profil d'ADN

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 9
D5S818: 10,13
D7S820: 11
THO1: 8,9
TPOX: 8,10
vWA: 17,18

Milieu de culture

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Autres remarques

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