MA104

85102918, African green monkey kidney, Epithelial

• Code UNSPSC : 41106514
• eCl@ss : 32190102

Propriétés

• product name: MA104, from African green monkey kidney, 85102918, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• Source biologique: African green monkey kidney
• Mode de croissance: Adherent
• Caryotype: Not specified
• Morphologie: Epithelial
• Récepteurs: Not specified

Description

Origine de la lignée cellulaire

Monkey African Green kidney

Description de la lignée cellulaire

Established from an African green monkey foetal kidney. The cells are highly susceptible to Simian rotavirus SA11.

Please note: The species of origin of the MA104 cell line has been shown to be different from that claimed by the originators.

Whitaker & Hayward (1985) found the cell line to originate from an African green monkey and not a Rhesus macaque as was originally claimed. Whitaker AM and Hayward CJ (1985). The characterization of three monkey kidney cell lines. Develop. Biol. Standard. Vol 60: 125-131. PMID: 4043530. Abstract: Three monkey kidney cell lines, Vero, GL-V3 and MA-104 were subjected to karyological analysis to determine their chromosomal stability and to confirm their species of origin. Although the lines were shown to be relatively stable throughout all of the passage levels that were tested, the species of origin of one of them was found to be different from that claimed by the originators. This finding was supported by data from isoenzyme studies.

Milieu de culture

EMEM (EBSS) (M2279) + 2 mM L-Glutamine (G7513) + 1% NEAA (M7145) + 10% FBS / FCS (F2442).

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Autres remarques

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