MDCK I

62106, dog kidney, Polarised epithelial

• Synonyme(s) : MDCK1 Cells, MDCKI Cells, Madin Darby Canine Kidney Cells, NBL-2 Cells
• Code UNSPSC : 41106514
• eCl@ss : 32190102

Propriétés

• product name: MDCK I, 00062106
• Source biologique: dog kidney
• Mode de croissance: Adherent
• Caryotype: Dog
• Morphologie: Polarised epithelial
• Produits: Not specified
• Récepteurs: Not specified
• Technique(s): cell culture | mammalian: suitable
• Conditions d'expédition: dry ice
• Température de stockage: −196°C

Description

Origine de la lignée cellulaire

Canine Cocker Spaniel kidney

Description de la lignée cellulaire

MDCK I - Madin-Darby canine kidney- is a subclone derived from the heterogeneous parent line MDCK (Madin-Darby canine kidney, Sigma Catalogue number. 85011435). MDCK-I cells, also known as "low passage MDCK" are reported to display a high electrical resistance (over 1000 Ohm/cm²) and form a monolayer with characteristics of tight epithelia of the renal collecting duct. Grown on filters, the cells show a cuboidal polarised morphology and express microvillae-like projections on the apical surface. Cultures on glass possess junctions but no polarised or columnar morphology. MDCK-I cells display an adrenalin, vasopressin and prostoglandin E sensitive adenyl cyclase. Please note, these cells were mycoplasma erradicated prior to deposit at ECACC. Epithelial phenotype may be unstable. Incomplete harvesting at confluence may select for smooth-muscle phenotype this is important if cells being used for transepithelial resistance studies.

Application

Virus susceptibility: Influenza virus, vesicular stomatitis virus and many other viruses.

Milieu de culture

EMEM (EBSS) (M2279) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Procédure de repiquage

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000cells/cm² using 0.25% tryspin or trypsin/EDTA; 5% CO₂; 37°C

Autres remarques

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