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Merck
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Principaux documents

AB0100

Sigma-Aldrich

Alkaline Phosphatase Blue Microwell Substrate

alkaline phosphatase substrate, solution

Synonyme(s) :

5-bromo-4-chloro-3-indolyl phosphate analog / NBT substrate for microwell assays

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.83

Nom du produit

Alkaline Phosphatase Blue Microwell Substrate, sufficient for 400 mL working substrate

Forme

solution

Niveau de qualité

200

Utilisation

sufficient for 400 mL working substrate

Température de stockage

2-8°C

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Description générale

Alkaline phosphatase blue microwell substrate, a two-component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require an insoluble reaction product.

Application

Prior to reaction with alkaline phosphatase, the BCIP® reagent is colorless to faint blue solution, and the Nitro BT reagent is a yellow solution. The two component mixture develops a bluish-purple product when reacted with alkaline phosphatase in microwell type assays. This substrate is not recommended for membrane or immunohistochemical type assays that require a precipitation reaction product.

Forme physique

A 2 component kit sufficient for preparing 400 mL of working substrate.
Supplied as a 2 component buffered alkaline phosphatase substrate containing Bromo-Chloro-Indolylphosphate (BCIP) and Nitro Blue Tetrazolium (NBT/Thiazolyl Blue/Nitro BT)

Informations légales

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

10 - Combustible liquids

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Certificats d'analyse (COA)

Lot/Batch Number
SLCP3050
SLCM1719
SLCN5760
SLCP2519
SLCM7961

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Sebastiano Giallongo et al.
Stem cells (Dayton, Ohio), 40(1), 35-48 (2022-05-06)
DNA damage repair (DDR) is a safeguard for genome integrity maintenance. Increasing DDR efficiency could increase the yield of induced pluripotent stem cells (iPSC) upon reprogramming from somatic cells. The epigenetic mechanisms governing DDR during iPSC reprogramming are not completely
Debojyoti Chakraborty et al.
Cell reports, 21(11), 3012-3021 (2017-12-16)
Long noncoding RNAs (lncRNAs) have been implicated in diverse biological processes, including embryonic stem cell (ESC) maintenance. However, their functional mechanisms remain largely undefined. Here, we show that the lncRNA Panct1 regulates the transient recruitment of a putative X-chromosome-encoded protein
Sarka Jelinkova et al.
Stem cell research, 40, 101562-101562 (2019-09-19)
Duchenne muscular dystrophy (DMD) affects 1:3500-5000 newborn boys and manifests with progressive skeletal muscle wasting, respiratory failure and eventual heart failure. Symptoms show different onset from patients' childhood to the second decade of age. We reprogrammed fibroblasts from two independent
Tandrima Mitra et al.
Mutation research, 832-833, 41-51 (2018-07-31)
The present study was undertaken to investigate the alterations in gene expression patterns and for mutation analysis of p53 in the riverine catfish Rita rita collected from polluted riverine habitat. The partial p53 gene sequence of Rita rita generated showed
Overexpression screen of chromosome 21 genes reveals modulators of Sonic hedgehog signaling relevant to Down syndrome.
Moyer, et al.
Disease models & mechanisms, 16 (2023)

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

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