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A4335

Sigma-Aldrich

Monoclonal Anti-Myosin (Skeletal, Fast)−Alkaline Phosphatase antibody produced in mouse

clone MY-32, purified from hybridoma cell culture

Synonyme(s) :

Monoclonal Anti-Myosin (Skeletal, Fast) antibody produced in mouse

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About This Item

Numéro MDL:
MFCD00145920
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.41

Source biologique

mouse

Conjugué

alkaline phosphatase conjugate

Forme d'anticorps

purified immunoglobulin

Type de produit anticorps

primary antibodies

Clone

MY-32, monoclonal

Forme

buffered aqueous glycerol solution

Espèces réactives

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

Technique(s)

direct immunofluorescence: 1:150 using formalin-fixed, paraffin-embedded human or animal skeletal muscle sections

Isotype

IgG1

Numéro d'accès UniProt

P12882

Conditions d'expédition

wet ice

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

Informations sur le gène

human ... MYH1(4619) , MYH2(4620)
mouse ... Myh1(17879) , Myh2(17882)
rat ... Myh1(287408) , Myh2(691644)

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Description générale

Localizes an epitope on the myosin heavy chain. Stains the fast (type II) and neonatal isomyosin molecules found in skeletal muscle, but does not stain cardiac muscle, smooth muscle or non-muscle myosin in cultured cells. Does react with human rhabdomyosarcomas.
Monoclonal Anti-Myosin (Skeletal, Fast) (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Spécificité

Monoclonal Anti-Myosin (Skeletal, Fast) specifically binds to an epitope in adult rat skeletal myosin heavy chains IIa, IIb and IId (IIx) (in fast-twitch fibers)†. The antibody product is also reactive in humans, bovines, cats, rabbits, mice, guinea pigs and chickens.

Immunogène

rabbit muscle myosin.

Application

Monoclonal Anti-Myosin (Skeletal, Fast) Alkaline Phosphatase antibody produced in mouse has been used for the detection and localization of skeletal muscle fast and neonatal myosins using immunohistochemistry. It may be used in muscle fiber typing, in studies of in vivo and in vitro muscle development and in the diagnosis of rhabdomyosarcomas.
The level of mysosin (fast) in serum samples from sportsmen with past injury was determined by western blot using monoclonal mouse anti-myosin (skeletal/fast) as the primary antibody at a dilution of 1:90000.

Actions biochimiques/physiologiques

Myosin (Skeletal, Fast) is useful not only in fiber typing but also in detection of myogenic tumors. It enables retrospective as well as prospective fiber typing using formalin-fixed, paraffin-embedded material.
Myosins are motor proteins that interact with actin filaments to regulate cell movement. Myosins also modulate cell shape and signaling. Skeletal muscle myosins have three ′fast′ myosin heavy chains isoforms (IIa, IIx, and IIb), a ′slow′ beta-myosin heavy chain isoform and three major myosin light-chain (MLC) isoforms. These myosin isoforms regulate skeletal muscle shortening.

Forme physique

Solution in 0.05 M Tris buffer, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl2, 50% glycerol, and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

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Vivi F H Jensen et al.
Basic & clinical pharmacology & toxicology, 122(1), 165-175 (2017-08-18)
Peripheral neuropathy is one of the most common complications of diabetic hyperglycaemia. Insulin-induced hypoglycaemia (IIH) might potentially exacerbate or contribute to neuropathy as hypoglycaemia also causes peripheral neuropathy. In rats, IIH induces neuropathy associated with skeletal muscle changes. Aims of
Haruhiko Kondoh et al.
Cardiovascular research, 69(2), 466-475 (2006-01-21)
Cell therapy is a promising strategy for ischemic cardiomyopathy. However, the direct injection method has limitations for generalized cell delivery, especially in dilated cardiomyopathy (DCM). We hypothesized that a sheet-shaped myoblast graft would be superior to direct injection for improving
Katy C Liu et al.
Bioarchitecture, 2(5), 158-170 (2012-09-08)
The development of cell-cell junctions was a fundamental step in metazoan evolution, and human health depends on the formation and function of cell junctions. Although it has long been known that actin and conventional myosin have important roles in cell
S Schiaffino et al.
Journal of applied physiology (Bethesda, Md. : 1985), 77(2), 493-501 (1994-08-01)
Skeletal muscles of different mammalian species contain four major myosin heavy-chain (MHC) isoforms: the "slow" or beta-MHC and the three "fast" IIa-, IIx-, and IIb-MHCs; and three major myosin light-chain (MLC) isoforms, the "slow" MLC1s and the two "fast" MLC1f
Glenda Comai et al.
Developmental cell, 31(5), 654-667 (2014-12-10)
The myogenic regulatory genes Myf5, Mrf4, Myod, and Myogenin likely arose by gene duplications during evolution, presumably to address the more demanding requirements of the vertebrate body plan. Two cell lineages were proposed to be regulated independently by Myf5 and
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