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47608

Sigma-Aldrich

Formaldéhyde solution

for molecular biology, BioReagent, ≥36.0% in H2O (T)

Synonyme(s) :

Formol

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About This Item

Formule linéaire :
HCHO
Numéro CAS:
50-00-0
Poids moléculaire :
30.03
Numéro Beilstein :
1209228
Numéro MDL:
MFCD00003274
Code UNSPSC :
12352114
ID de substance PubChem :
329757328
Nomenclature NACRES :
NA.52

Qualité

for molecular biology

Niveau de qualité

100

Densité de vapeur

1.03 (vs air)

Pression de vapeur

52 mmHg ( 37 °C)

Gamme de produits

BioReagent

Forme

liquid

Température d'inflammation spontanée

572 °F

Contient

~10% methanol as stabilizer

Concentration

≥36.0% in H2O (T)
36.0-38.0% (aldehyde method)

Technique(s)

electrophoresis: suitable

Impuretés

≤0.03% free acid (as HCOOH)

Résidus de calcination

≤0.05% (as SO4)

pH

2.8-4.0

Densité

1.09 g/mL at 20 °C
1.09 g/mL at 25 °C (lit.)

Traces d'anions

chloride (Cl-): ≤10 mg/kg
sulfate (SO42-): ≤20 mg/kg

Traces de cations

Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Na: ≤100 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Zn: ≤1 mg/mL

λ

2 M in H2O

Absorption UV

λ: 260 nm Amax: 0.01
λ: 280 nm Amax: 0.01

Adéquation

in accordance for electrophoresis of RNA in agarose gels

Température de stockage

room temp

Chaîne SMILES 

[H]C([H])=O

InChI

1S/CH2O/c1-2/h1H2

Clé InChI

WSFSSNUMVMOOMR-UHFFFAOYSA-N

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Description générale

Formaldehyde is a molecular biology reagent that denaturates RNA during electrophoresis when incorporated in agarose gels.
It is an aliphatic aldehyde that is widely used in industrial applications for the synthesis of several chemicals such as melamine formaldehyde, urea-formaldehyde, phenol formaldehyde and industrial resins, paraformaldehyde, polyols, chelating agents, slow release fertilizers, 1,4-butanediol and hexamethylenetetramine.

Application

Formaldehyde solution is suitable for:
  • denaturation and separation of RNA molecules
  • anti-bacterial treatment
  • fixation of cells
Formaldehydesolution has been used:
  • in the fixation of human spermatogonial stem cell transplantation (SSCT).
  • to crosslink T- cell acute lymphoblastic leukemia protein/DNA complex during chromatin immunoprecipitation.
  • in fixing zebra fish larvae for neutrophil staining.

Attention

may contain precipitate (paraformaldehyde)

Principe

Formaldehyde disrupts the secondary structure of RNA and allows separation and identification of RNA species based on charge migration.

Produit(s) apparenté(s)

Réf. du produit
Description
Tarif

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3

Organes cibles

Eyes,Central nervous system, Respiratory system

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

133.0 °F - closed cup

Point d'éclair (°C)

56.11 °C - closed cup

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation
Onofre J, et al.
Reproductive Biomedicine Online, 40(2), 261-269 (2020)
Formaldehyde
Gerberich, H R and Seaman, G C.
Kirk-Othmer Encyclopedia of Chemical Technology (2013)
Jin Qian et al.
Virology, 474, 10-18 (2014-12-03)
Interferon-induced transmembrane (IFITM) proteins inhibit the entry of a large number of viruses. Not surprisingly, many viruses are refractory to this inhibition. In this study, we report that different strains of HIV and SIV are inhibited by human IFITM proteins
Dannis G van Vuurden et al.
Neuro-oncology, 16(7), 946-959 (2014-01-29)
Glial brain tumors cause considerable mortality and morbidity in children and adults. Innovative targets for therapy are needed to improve survival and reduce long-term sequelae. The aim of this study was to find a candidate tumor-promoting protein, abundantly expressed in
Y Jiang et al.
Oncogene, 34(50), 6079-6091 (2015-03-10)
Epstein-Barr virus (EBV) causes human lymphoid malignancies, and the EBV product latent membrane protein 1 (LMP1) has been identified as an oncogene in epithelial carcinomas such as nasopharyngeal carcinoma (NPC). EBV can epigenetically reprogram lymphocyte-specific processes and induce cell immortalization.
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