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Key Documents

P7949

Sigma-Aldrich

TWEEN® 20

BioXtra, viscous liquid

Synonyme(s) :

Monolaurate de polyoxyéthylènesorbitan, Monolaurate de polyéthylèneglycolsorbitan

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About This Item

Numéro CAS:
Numéro MDL:
Code UNSPSC :
12161900
Nomenclature NACRES :
NA.21

Source biologique

plant

Niveau de qualité

Description

non-ionic

Gamme de produits

BioXtra

Forme

viscous liquid

Poids mol.

~1228

Composition

lauric acid, ≥40% (balance primarily myristic, palmoyic, and stearic acids)

Technique(s)

blocking: suitable
cell culture | mammalian: suitable
hybridization: suitable
protein purification: suitable
transformation: suitable

Impuretés

≤0.05% Phosphorus (P)

Résidus de calcination

≤0.2%

Indice de réfraction

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

Température de transition

cloud point 76 °C

Densité

1.095 g/mL at 25 °C (lit.)

Traces d'anions

chloride (Cl-): ≤0.05%
sulfate (SO42-): ≤0.05%

Traces de cations

Al: ≤0.0005%
Ca: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Mg: ≤0.0005%
NH4+: ≤0.05%
Na: ≤0.05%
Pb: ≤0.001%
Zn: ≤0.0005%

HLB

16.7

Chaîne SMILES 

CCCCCCCCCCCC(=O)OCCOCC(C1C(C(CO1)OCCO)OCCO)OCCO

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

Clé InChI

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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Description générale

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Tween® 20 is a nonionic surfactant and detergent widely applied in biochemical and life science research. Its predominant use involves the solubilization of membrane proteins during the isolation of membrane-protein complexes. Moreover, it serves as a component in various washing, blocking, dilution, and stabilization buffers. Additionally, it functions as a reagent for immunohistochemistry, encompassing techniques like Western blotting and ELISAs. In these applications, Tween® 20 plays crucial roles such as preventing non-specific antibody binding, minimizing background staining, and ensuring the even distribution of reagents.

Surfactants, characterized by hydrophilic and hydrophobic elements, are broadly classified as either ionic (anionic, cationic, or ampholytic) or nonionic, depending on their ionization behavior in water. The specific product in question falls into the nonionic category and finds versatile applications, including emulsification, dispersion, solubilization, and as a detergent. Notably, it can also act as a solubilizer for membrane proteins.

Application

TWEEN 20 has been used in post-hybridization wash buffer after in situ hybridization. It has been used along with PBS (phosphate buffered saline) to wash wells during ELISA and along with PBS (phosphate buffered saline) in wash buffer for immunostaining.
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Détergent non ionique

Caractéristiques et avantages

  • Highly versatile surfactant for your Life Science and Biochemical research
  • BioXtra grade for your sensitive applications
  • Tested for trace levels of Anions and Cations
  • Excellent solubilizing, dispersing, and emulsifying agent
  • Suitable for mammalian cell culture, protein purification, and hybridization studies

Attention

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Notes préparatoires

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Autres remarques

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Informations légales

TWEEN is a registered trademark of Croda International PLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

527.0 °F - Pensky-Martens closed cup

Point d'éclair (°C)

275 °C - Pensky-Martens closed cup

Équipement de protection individuelle

Eyeshields, Gloves


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Consulter la Bibliothèque de documents

Coexpression of the cannabinoid receptor type 1 with dopamine and serotonin receptors in distinct neuronal subpopulations of the adult mouse forebrain.
Hermann H, et al.
Neuroscience, 109(3), 451-460 (2002)
Localizing transcription factors on chromatin by immunofluorescence.
Pile LA and Wassarman DA
Methods, 26(1), 3-9 (2002)
Comparison of the results obtained by ELISA and surface plasmon resonance for the determination of antibody affinity.
Heinrich L, et al.
Journal of Immunological Methods, 352(1-2), 13-22 (2010)
Production of hen egg yolk immunoglobulins simultaneously directed against Salmonella Enteritidis and Salmonella Typhimurium in the same egg yolk.
Chalghoumi R, et al.
Poultry Science, 87(1), 32-40 (2008)
Jingyu Yao et al.
Autophagy, 12(12), 2439-2450 (2016-10-30)
Autophagy is a lysosomal degradation pathway critical to preventing the accumulation of cytotoxic proteins. Deletion of the essential autophagy gene Atg5 from the rod photoreceptors of the retina (atg5Δrod mouse) results in the accumulation of the phototransduction protein transducin and

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