The determination of the pH of Toluidine Blue powder in distilled water is not a quality specification. Section 9 of the Safety Data Sheet states the pH data is not available. It is reported elsewhere in the literature that the powder has a pH of between 2 and 2.5, but this information has not been confirmed for product 89640. The literature also reports the pKA for toluidine blue is around pH 4.5, but there are other pKa values reported in the literature. The pH of distilled water is typically around 4.2. Toluidine Blue has various uses. Some procedures call for dissolving the Toluidine Blue in water, but procedures that recommend diluting in various alcohol concentrations are also common.
89640
Toluidine Blue
suitable for microscopy, 8.74% (ZN (THEORY))
Synonyme(s) :
Tolonium chloride; Basic Blue 17
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A propos de cet article
Formule empirique (notation de Hill) :
C15H16ClN3S · 0.5ZnCl2
Numéro CAS:
Poids moléculaire :
373.97
PubChem Substance ID:
UNSPSC Code:
12171500
NACRES:
MA.02
MDL number:
Colour Index Number:
52040
Beilstein/REAXYS Number:
4068052
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Service technique
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Laissez-nous vous aiderQuality Level
assay
8.74% (ZN (THEORY))
form
powder or crystals
technique(s)
microbe id | staining: suitable
color
green to very dark green
solubility
methanol: 0.01 g/10 mL, blue to very deep blue
εmax
≥800 at 621-636 nm in water
suitability
suitable for microscopy
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
[Cl-].[Cl-].Cl[Zn]Cl.Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C.Cc4cc5N=C6C=CC(\C=C6Sc5cc4N)=[N+](\C)C
InChI
1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2
InChI key
DAQXTSXITPUGRG-UHFFFAOYSA-L
General description
Toluidine blue (C.l. 52040) is a diaminothiazine dye.It is also known as TBO, tolonium chloride, and toluidine blue O.
Application
Toluidine Blue is suitable for use in the following applications:
- to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
- in metachromatic staining of sulfated mucins and related materials
- as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
- staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)
Biochem/physiol Actions
Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nuclear material of tissues with a high DNA and RNA content.
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Cet article | |||
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - |
| suitability suitable for microscopy | suitability - | suitability suitable for microscopy | suitability suitable for microscopy |
| color green to very dark green | color - | color - | color - |
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| solubility methanol: 0.01 g/10 mL, blue to very deep blue | solubility H2O: 1 mg/mL | solubility water: 0.01 g/10 mL, blue to very deep blue | solubility toluene: 0.01 g/10 mL, blue to very deep blue |
| form powder or crystals | form powder | form powder or crystals | form powder |
Classe de stockage
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Numéro d'article de commerce international
| Référence | GTIN |
|---|---|
| 89640-5G | 04061835316243 |
| 89640-25G | 04061833076385 |
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What about the basic PH value of Toluidine Blue powder (89640) mixing with distilled water?
1 answer-
Helpful?
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Are there any instructions on how to mix Toluidine Blue powder (89640) into a staining solution?
1 answer-
Toluidine Blue is used in various staining procedures, and preparation may vary depending on the specific procedure. For staining frozen sections, aqueous solutions with concentrations ranging from 0.3% to 1% can be used. Additionally, a 0.5% dye in 20% alcohol is suitable for frozen sections. Other solutions may require a specific pH or special preparation.
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What does C.l. 52040 mean?
1 answer-
The C.I. number is the Colour Index number. This number is globally recognized and is assigned to Dyes and Pigments by the Americal Association of Textile Chemists and Colourists (AATCC) established in 1924. This compound is assigned a C.I. number of 52040. Please see the link below to learn more about the Colour Index program:
https://colour-index.com/Helpful?
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