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Key Documents

CSPD-RO

Roche

CSPD ready-to-use

Disodium 3-(4-methoxyspiro {1,2-dioxetane-3,2′-(5′-chloro)tricyclo [3.3.1.13,7]decan}-4-yl)phenyl phosphate

Synonyme(s) :

ready-to-use, cspd

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About This Item

Code UNSPSC :
41105500

Niveau de qualité

100

Pureté

>98% (HPLC)

Forme

solution

Poids mol.

Mr 461

Conditionnement

pkg of 1 mL (11655884001[25mM])
pkg of 2 × 50 mL (11755633001[0.25mM] ready-to-use)

Fabricant/nom de marque

Roche

Température de stockage

2-8°C

Description générale

CSPD is a chemiluminescent substrate for alkaline phosphatase that enables extremely fast and sensitive detection of biomolecules by producing visible light. Light emission is recorded on X-ray film or on luminescence imager systems.

Application

CSPD ready-to-use has been used for:
  • the nonradioactive detection of labeled nucleic acid probes on membrane blots (Southern blot, Northern blot, Western blot and Dot blot)
  • colony and plaque hybridization
  • gel shift assay
  • sequencing
It may be used for the detection of alkaline phosphatase and alkaline phosphatase conjugates either in solution or on solid supports.

Caractéristiques et avantages

  • Save time with a fast, highly sensitive substrate.
  • Easily strip and reprobe membranes.
  • Take multiple exposures of up to two days for each experiment.
  • Reuse the substrate by filtering and storing in sodium azide.

Forme physique

25mM solution (11.6mg/ml), 100x concentrated, colorless solution.

Notes préparatoires

Storage conditions (working solution): Washing Buffer: 15 to 25 °C
Maleic Acid Buffer: 15 to 25 °C
Detection Buffer: 15 to 25 °C
Blocking Stock Solution, 10x: 2 to 8 °C
Blocking Solution: always prepare fresh
Antibody Solution: 2 to 8 °C

Autres remarques

For life science research only. Not for use in diagnostic procedures.
Purity: Purified by HPLC
Development time: Variable
Note: First exposure to X-ray film should be 15 to 30 minutes. Examine film and then determine other exposure times based on initial result.
Activated by preincubation (10 minutes at +37°C).
Apply substrate quickly to avoid drying of membrane (background).
Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film.
Do not use nitrocellulose membranes.
Repeat exposures can be made several days after addition of substrate.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

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Consulter la Bibliothèque de documents

C S Martin et al.
BioTechniques, 18(5), 908-913 (1995-05-01)
Quantitative PCR and reverse transcription PCR (RT-PCR) are widely used in biomedical, industrial and other research applications to determine the number of RNA or DNA molecules of a specific type and/or sequence in a sample of interest. We have developed
Hoa Mai Nguyen et al.
Plant physiology, 163(2), 914-928 (2013-08-21)
The ω-3 polyunsaturated fatty acids account for more than 50% of total fatty acids in the green microalga Chlamydomonas reinhardtii, where they are present in both plastidic and extraplastidic membranes. In an effort to elucidate the lipid desaturation pathways in
Diane M Ramos et al.
BMC developmental biology, 6, 55-55 (2006-11-23)
Precise temporal and spatial regulation of transgene expression is a critical tool to investigate gene function in developing organisms. The most commonly used technique to achieve tight control of transgene expression, however, requires the use of specific DNA enhancers that

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