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Key Documents

11467140001

Roche

DNA, MB-grade

from fish sperm

Synonyme(s) :

DNA

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About This Item

Code UNSPSC :
41106310

Source biologique

fish (sperm)

Niveau de qualité

Qualité

Molecular Biology

Forme

solution (ready-to-use)

Poids mol.

40-1,000 kDa

Conditionnement

pkg of 500 mg

Fabricant/nom de marque

Roche

Concentration

100 μg/mL

Couleur

light yellowopalescent

Solubilité

water: miscible

Température de stockage

−20°C

Description générale

DNA, MB-grade is used for the prevention of nonspecific hybridization reactions.
DNA, MB-grade, is provided as a sonicated, denatured single-stranded DNA fragment mixture.

Application

The ready-to-use solution is directly added to the hybridization mix. It prevents nonspecific hybridization in all types of membrane (e.g., Southern and northern blot applications) or in situ DNA hybridizations. DNA, MB-grade has been used in chromatin immunoprecipitation (ChiP) assay.
Note: Addition of NaCl, sonication or shearing, and denaturing are not required.

Séquence

Chain Length 120 to 3,000 nucleotides

Forme physique

Solution, 10 mg/ml, in 10 mM Tris-HCl, 10 mM NaCl, 1 mM EDTA, pH 8.0

Notes préparatoires

Working concentration: 100μg/ml
This fish sperm DNA should be used at a concentration of 100 μg/ml (1 : 100 dilution) in prehybridization solutions.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

nwg

Point d'éclair (°F)

No data available

Point d'éclair (°C)

No data available


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