11119915001
Roche
RNase, DNase-free
from bovine pancreas
Synonyme(s) :
Rnase
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About This Item
Code UNSPSC :
41105600
Produits recommandés
Source biologique
bovine pancreas
Niveau de qualité
Forme
solution
Activité spécifique
≥30 units/mg protein
Conditionnement
pkg of 500 μg (1 ml)
Fabricant/nom de marque
Roche
Technique(s)
DNA purification: suitable
Température de stockage
−20°C
Description générale
Pyrimidine-specific endoribonuclease that acts on single-stranded RNA. RNase, DNase-free, is a heterogeneous mixture of ribonucleases that has been prepared free of deoxyribonuclease activity according to the current Quality Control procedures. RNase, DNase-free, is particularly well suited for use in DNA isolation procedures. Before use, most RNase preparations must be boiled to remove DNase activity. This preparation of RNase does not need to be boiled; it can be used directly from the vial.
Application
RNase, DNase-free, efficiently removes contaminating RNA from plasmid or genomic DNA preparations.
Définition de l'unité
One Kunitz unit is the amount of enzyme that causes a decrease in absorbance of A0 to A1 within one minute under the assay conditions. A0 to A1 corresponds to the total conversion, A1 being the final absorbance.
One unit produces a decrease in absorbance at 260 nm, which is equivalent to a total conversion of RNA to oligonucleotides in one minute at +25 °C.
One unit produces a decrease in absorbance at 260 nm, which is equivalent to a total conversion of RNA to oligonucleotides in one minute at +25 °C.
Forme physique
Solution, 500 μg/ml, in 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (pH 7.0).
Notes préparatoires
Working concentration: The optimal working concentration for RNase, DNase free, is 2 to 5 μg/ml. The reaction volume will vary for different applications. Some suggested guidelines are given below:
Working solution: Storage and Dilution Buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0.
- For small-scale isolation of plasmid DNA ("miniprep" from a 1.5 ml bacterial culture), use 0.5 μl of RNase, DNase-free in a reaction volume of 50 μl.
- To isolate plasmid DNA from a 100 ml bacterial culture, use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
- To isolate genomic DNA from cultured mammalian cells (5 x 107 cells), use 8 μl of RNase, DNase-free in a reaction volume of 2 ml.
Working solution: Storage and Dilution Buffer: 10 mM Tris-HCl, 5 mM CaCl2, 50% glycerol (v/v), pH 7.0.
Autres remarques
For life science research only. Not for use in diagnostic procedures.
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
No data available
Point d'éclair (°C)
No data available
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Protocoles
0.1 mU RNase, DNase-free degrades 1 μg RNA in 30 min at + 37 °C in a reaction volume of 50 μL PCR grade water. The protein concentration of RNase, DNase-free is 0.5 μg/μL.
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