Accéder au contenu
Merck
Toutes les photos(1)

Key Documents

View All Documentation

10220647001

Roche

GTP-γ-S

Tetralithium salt

Synonyme(s) :

GTP-γ-S, GTP

Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Toutes les photos(1)

About This Item

Code UNSPSC :
12352204

Description

C10H12N5O13P3SLi4

Niveau de qualité

100

Pureté

87% (HPLC)

Forme

solid

Poids mol.

Mr 539.2 (GTP-γ-S)
Mr 563.0 (GTP-γ-S-Li4)

Composition

GDP, ≤12% HPLC
GMP, ≤1% HPLC
GTP, ≤2% HPLC

Conditionnement

pkg of 10 mg

Fabricant/nom de marque

Roche

Conditions de stockage

protect from light

Concentration

97%

Technique(s)

activity assay: suitable

Couleur

white

Solubilité

water: soluble

Température de stockage

−20°C (−15°C to −25°C)

Description générale

Guanosine 5′-O-(3-thiotriphosphate), tetralithium salt (GTP-γ-S) is a non-hydrolysable analog of GTP. It is an inhibitor of phosphodiesterase in photoreceptors.

Application

GTP-γ-S has been used:
  • in G-Protein activation assay to assess the functionality of protease-activated receptor 4 (PAR4)
  • in fluorimetric guanine nucleotide exchange assay of G protein alpha subunits (Gα)
  • to monitor the Rac family small guanosine triphosphatase (GTPase) 2 (Rac2)-stimulated activity of phospholipase Cγ2 mutants

Actions biochimiques/physiologiques

Guanosine 5′-O-(3-thiotriphosphate) (GTP-γ-S) activates guanine-nucleotide-binding proteins and is slowly hydrolyzed enzymatically. It inhibits guanosine triphosphatase (GTPases) more potently than guanosine triphosphate (GTP),

Stockage et stabilité

Store powder dry at -15 to -25 °C; aqueous solutions with pH approx. 7 should be stable for six months at -15 to -25 °C.

Remarque sur l'analyse

Absorption: Content is measured in aqueous solution by absorption at 254 nm.
Conditions:
  • approx. 10 mg/ml double-dist. water
  • dilution: 9.8 ml double-dist. water + 0.2 ml sample.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

Déjà en possession de ce produit ?

Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

Gilbert Di Paolo, Markus R. Wenk
Lipids, 3-18 (2012)
Stephen R Sprang
Biopolymers, 105(8), 449-462 (2016-03-22)
This review addresses the regulatory consequences of the binding of GTP to the alpha subunits (Gα) of heterotrimeric G proteins, the reaction mechanism of GTP hydrolysis catalyzed by Gα and the means by which GTPase activating proteins (GAPs) stimulate the
S Nawy et al.
Neuron, 7(4), 677-683 (1991-10-01)
Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement
K Sasaki et al.
Nature, 325(6101), 259-262 (1987-01-15)
Recently, a GTP-binding protein sensitive to islet activating protein (IAP) has been suggested to be important in producing K+-currents when the muscarinic receptor of the atrial muscle is activated by acetylcholine (ACh). Here we confirm the blocking effects of IAP
Baisen Zeng et al.
Structure (London, England : 1993), 27(7), 1137-1147 (2019-06-04)
Ric-8A is a 530-amino acid cytoplasmic molecular chaperone and guanine nucleotide exchange factor (GEF) for i, q, and 12/13 classes of heterortrimeric G protein alpha subunits (Gα). We report the 2.2-Å crystal structure of the Ric-8A Gα-binding domain with GEF
Afficher tous les articles scientifiques apparentés

Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..

Contacter notre Service technique