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10106399001

Roche

GTP

>90% (HPLC), crystals, pkg of 250 mg

Synonyme(s) :

Guanosine 5′-triphosphate sodium salt hydrate, 5′-GTP-Na2

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About This Item

Formule empirique (notation de Hill):
C10H16N5O14P3 · xNa+ · yH2O
Numéro CAS:
Poids moléculaire :
523.18 (anhydrous free acid basis)
Numéro Beilstein :
4113439
Numéro MDL:
Code UNSPSC :
41106305
ID de substance PubChem :

Description

Disodium salt

Niveau de qualité

Pureté

>90% (HPLC)

Forme

crystals

Poids mol.

Mr 523.2 (GTP)
Mr 567.1 (GTP-Na2)

Conditionnement

pkg of 250 mg

Fabricant/nom de marque

Roche

Conditions de stockage

avoid repeated freeze/thaw cycles

Chaîne SMILES 

O.O.[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1

InChI

1S/C10H16N5O14P3.2Na.2H2O/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;2*1H2/q;2*+1;;/p-2/t3-,5-,6-,9-;;;;/m1..../s1

Clé InChI

HPLNHYUZFVUXFJ-ZVQJTLEUSA-L

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Description générale

GTP functions as a carrier of phosphates and pyrophosphates involved in channeling chemical energy into specific biosynthetic pathways. It also serves as an energy-rich precursor of mononucleotide units in the enzymatic biosynthesis of DNA and RNA. GTP is involved in the initiation of peptide synthesis during the binding of formylmethionyl-tRNA (F-met-tRNA) to the ribosome. It is also involved in polypeptide chain elongation. GTP is hydrolyzed by tubulin and its hydrolysis is accompanied by microtubule assembly.
GTP is supplied as the disodium salt in crystal form.

Application

GTP, disodium salt, is required as a coenzyme for protein biosynthesis in a cell-free system. It has been used in FtsZ polymerization assay and in the preparation of pipette solution for the electrophysiological recordings of the whole-cell membrane currents.

Qualité

Purity: 76% GTP (enzymatic), >90 area% (HPLC), 9% Na+, 8% H2O

Notes préparatoires

Activator: EDTA prevents the formation of enzyme aggregates. Phosphate stimulates the NADH-dependent reaction.
Storage conditions (working solution): Aqueous solutions (pH 7.0) are stable at 2 to 8 °C or frozen for approx. one week, for reasons of hydrolysis pH should not be < 3.

If neutralized with water preparation can be stored frozen at -15 to -25 °C in small portions, one sample should thawed only once and should be used within the next one or two days (stored at 2 to 8 °C). Preparation can also be dissolved in a buffer pH 7-8 without neutralization (buffer concentration should be high enough that pH is not changed by addition of those substances); solution can be stored frozen.

Investigations on the storage temperature 2 to 8 °C compared to -15 to -25 °C showed no difference.

Stockage et stabilité

Store at 2 to 8 °C. (Store dry!)

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Alice Cezanne et al.
eLife, 9 (2020-06-09)
Proteins can self-organize into spatial patterns via non-linear dynamic interactions on cellular membranes. Modelling and simulations have shown that small GTPases can generate patterns by coupling guanine nucleotide exchange factors (GEF) to effectors, generating a positive feedback of GTPase activation
Ewa Król et al.
Journal of visualized experiments : JoVE, (81)(81), e50844-e50844 (2013-12-05)
During bacterial cell division, the essential protein FtsZ assembles in the middle of the cell to form the so-called Z-ring. FtsZ polymerizes into long filaments in the presence of GTP in vitro, and polymerization is regulated by several accessory proteins.
Lehninger, A.L
Biochemistry, 316-316 (1977)
The role of guanosine 5'-triphosphate in polypeptide chain elongation.
Y Kaziro
Biochimica et biophysica acta, 505(1), 95-127 (1978-09-21)
Marzia Govoni
Bio-protocol, 7(3), e2120-e2120 (2017-02-05)
In eukaryotic cells transcriptional processes are carried out by three different RNA polymerases: RNA polymerase I which specifically transcribes ribosomal RNA (rRNA), RNA polymerase II which transcribes protein-coding genes to yield messenger RNAs (mRNAs) and small RNAs, while RNA polymerase

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