MABN695

Anti-CRTC2 Antibody, clone 5B10

ascites fluid, clone 5B10, from mouse

• Synonyme(s) : TORC2, TORC-2, CRTC2, CREB-regulated transcription coactivator 2, Transducer of regulated cAMP response element-binding protein 2, Transducer of CREB protein 2
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: ascites fluid
• Type de produit anticorps: primary antibodies
• Clone: 5B10, monoclonal
• Espèces réactives: human
• Technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotype: IgG1
• Numéro d'accès NCBI: NP_859066
• Numéro d'accès UniProt: Q53ET0
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... CRTC2(200186)

Description

Description générale

CREB-regulated transcription coactivator 2 (CRTC2) is a transcriptional coactivator for CREB1 which is a cAMP (CRE) site responder. CRTC2 is also known as TORC-2 (transducer of regulated cAMP response element-binding protein 2) and CRTC2 regulates gluconeogenesis as well as steroidogenic processes, and is also a coactivator for mitochondrial biogenesis in muscle cells. Localization is cytoplasmic but relocates to the nucleus in response to cAMP and glucagon levels. CRTC2 is highly expressed in thymus, spleen, muscle, and lung; it is also expressed in various cancers as well as insulinomas. Low levels of expression are found in the brain, colon, heart and kidney, with the lowest levels being in the liver and pancreas. Activity is regulated via phosphorylation. CRTC2 is inactive when phosphorylated (meaning that is does not bind CREB and assist in gene transcription) and active when dephosphorylated. EMD-Millipore’s Anti-CRTC2 mouse monoclonal, clone 5B10, has been tested in western blots against human HEK292 and HeLa cell extracts, in immunohistochemistry in paraffin embedded human ovary and lung cancer tissue, and in immunofluorescent immunocytochemistry and flow cytometry on HeLa cells in culture.

Immunogène

Purified protein fragment of human CRTC2 expressed in E. coli.

Application

Anti-CRTC2 Antibody, clone 5B10 is a highly specific mouse monoclonal antibody, that targets CREB-regulated transcription coactivator 2 & has been tested in western blotting, ICC, IHC & Flow Cytometry.

Immunocytochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC2 in HeLa cells.

Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CRTC2 in human lung cancer and ovarian tumor tissues.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CRTC2 in HeLa cells.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

Qualité

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CRTC2 in 10 µg of HeLa cell lysate.

Description de la cible

~76 kDa observed.

Forme physique

Mouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.

Unpurified

Stockage et stabilité

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Remarque sur l'analyse

Control
HeLa cell lysate

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable