MAB2600

Anti-Glypican-1 Antibody, clone 4D1

clone 4D1, from mouse

• Synonyme(s) : glypican 1, glypican proteoglycan 1
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: mouse
• Niveau de qualité: 100
• Forme d'anticorps: purified antibody
• Type de produit anticorps: primary antibodies
• Clone: 4D1, monoclonal
• Espèces réactives: human, rat
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotype: IgG1κ
• Numéro d'accès NCBI: NP_002072
• Numéro d'accès UniProt: P35052
• Conditions d'expédition: wet ice
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... GPC1(2817) ; rat ... Gpc1(58920)

Description

Description générale

Heparan sulfate proteoglycans (HSPGs) can be divided into general categories of
cell-associated forms, including syndecans, glypicans, and secreted extracellular matrix forms, such as perlecan. Glypicans contain six family members to date and are proteins bound to the outer surface of the plasma membrane by a glycosyl-phosphatidylinositol anchor and involved in cellular adhesion and migration and function as accessory molecules for signaling receptors, (Filmus, 2008 & Gui, 2006). Current publications indicate the main function of membrane-attached glypicans is to regulate the signaling of Wnts, Hedgehogs, fibroblast growth factors and bone morphogenetic proteins (BMPs), (Filmus, 2008).
Glypican-1 (GPC1) is over-expressed in human gliomas and appears to have a greater capability of promoting FGF-2 signaling in some gliomas versus HSPGs in normal astrocytes. Additional observations suggest that GPC1 can impact the growth of pancreatic cancer cells by attenuating tumor angiogenesis, (Whipple, 2008).

Spécificité

MAB2600 recognizes glypican-1.

Immunogène

Epitope: Full Length

Full length recombinant protein.

Application

Anti-Glypican-1 Antibody, clone 4D1 detects level of Glypican-1 & has been published & validated for use in IH(P) & WB.

Immunohistochemistry:
A 1:50 dilution of a previous lot detected strong cytoplasmic staining of tumor cells in formalin-fixed, paraffin-embedded gangliobastoma tissue sample. There was negative staining of normal, uninvolved cells.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Qualité

Routinely evaluated by Western blot on human PC-12 cell lysate using 0.5 µg/mL of Anti-Glypican-1 at 1 mg/mL.

Description de la cible

Recognizes Glypican-1 at ~ 59 kDa

Forme physique

Format: Purified

Protein G Purified

Purified in 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% NaN3.

Stockage et stabilité

Stable for 1 year at 2-8ºC from date of receipt.

Remarque sur l'analyse

Control
PC-12 cell lysate.

Autres remarques

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable