EZRGRT

Rat/Mouse Ghrelin ELISA Kit

measures and quantifies total Ghrelin in 20 μL serum or plasma

• Synonyme(s) : Appetite Regulating Hormone, Growth hormone-releasing peptide, Interleukin-6, Motilin-related peptide
• Code UNSPSC : 12161503
• eCl@ss : 32161000
• Nomenclature NACRES : NA.32

Propriétés

• product name: Rat/Mouse Ghrelin (total) ELISA, This Rat/Mouse Ghrelin (total) ELISA is used to measure & quantify Ghrelin (total) levels in Metabolism & Endocrine research.
• Niveau de qualité: 200
• Espèces réactives: mouse, rat
• Conditionnement: kit of 1 × 96 wells
• Paramètres: 20 μL sample volume (4hr assay)
• assay range: accuracy: 80-103%; (Serum: Active Ghrelin); accuracy: 86-102%; (Plasma: Active Ghrelin); accuracy: 92-103%; (Serum: Des-octanoyl Ghrelin); accuracy: 95-107%; (Plasma: Des-octanoyl Ghrelin); inter-assay cv: 1.8-4.5%; intra-assay cv: 0.7-1.3%; (Serum); inter-assay cv: 2.0-3.2%; intra-assay cv: 1.1-1.7%; (Plasma)
• Technique(s): ELISA: suitable
• Entrée: sample type plasma (K2 EDTA); sample type serum
• Numéro d'accès UniProt: Q9EQX0 (mouse); Q9QYH7 (rat); Q9UBU3
• Application(s): research use
• Méthode de détection: colorimetric (450nm/590nm)
• Conditions d'expédition: wet ice
• Température de stockage: 2-8°C

Description

Description générale

Circulating ghrelin is a multifunctional hormone secreted primarily by the stomach. Originally found as an endogenous ligand for the growth hormone secretagogue receptor, it is also an appetite-stimulating hormone in the regulation of energy homeostasis. It consists of 28 amino acids and the n-octanoylation of serine-3 position is necessary for its bioactivity. The acylated form is extremely unstable in the blood; consequently, the predominant form is des-octanoyl ghrelin. Long considered a key player in obesity, ghrelin may also play a role in maintaining the balance between insulin and glucose and the development of diabetes. The spike in insulin secretion occurring after eating usually correlates with a dip in ghrelin production. Researchers determined that in rat models of uncontrolled diabetes, food intake still suppressed ghrelin concentrations in the blood, but only approximately half as effectively as when insulin was present.

Spécificité

Canine Ghrelin (Active) - 71%
Human Ghrelin (Active) - 115%
Human Des-Octanoyl Ghrelin - 249%
Porcine Ghrelin (Active) - 48%
Rat/Mouse Ghrelin (Active) - 85%
Rat/Mouse Des-Octanoyl Ghrelin - 100%

Application

Research Category
Metabolism

Research Sub Category
Obesity

Metabolic Disorders

Room temperature, 3 hour assay, 20 μL sample size (serum or plasma)

Used to detect/quantify: Ghrelin (Total)

Autres remarques

Please contact Technical Service for linearity of dilution.

Clause de non-responsabilité

For research use only. Not for use in diagnostic procedures.

Informations sur la sécurité

• Pictogrammes: GHS06,GHS05,GHS09
• Mention d'avertissement: Danger
• Mentions de danger: H290,H302 + H332,H311,H315,H317,H319,H335,H411
• Conseils de prudence: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Classification des risques: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
• Organes cibles: Respiratory system
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects