ABS1487

Anti-P2Y12 Receptor

from rabbit, purified by affinity chromatography

• Synonyme(s) : P2Y purinoceptor 12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999
• Code UNSPSC : 12352203
• eCl@ss : 32160702
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Produit purifié par: affinity chromatography
• Espèces réactives: rat, human
• Conditionnement: antibody small pack of 25 μg
• Technique(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Numéro d'accès NCBI: NP_073625
• Numéro d'accès UniProt: Q9H244
• Modification post-traductionnelle de la cible: unmodified
• Informations sur le gène: human ... P2RY12(64805)

Description

Description générale

P2Y purinoceptor 12 (UniProt: Q9H244; also known as P2Y12, ADP-glucose receptor, ADPG-R, P2T(AC), P2Y(AC), P2Y(cyc), P2Y12 platelet ADP receptor, P2Y(ADP), SP1999) is encoded by the P2RY12 (also known as HORK3) gene (Gene ID: 64805) in human. P2Y12 is a multi-pass metabotropic receptor that is highly expressed in the platelets. Lower expression levels have been observed in the brain, lung, and adrenal glands. P2Y12 serves as a receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. It is not activated by UDP and UTP. It is required for normal platelet aggregation and blood coagulation. It is the predominant receptor involved in the ADP-stimulated activation of the glycoprotein IIb/IIIa receptor. Activation of the glycoprotein IIb/IIIa receptor results in enhanced platelet degranulation and thromboxane production, and prolonged platelet aggregation. P2Y12 receptor has also been reported to be expressed on microglia and is required to neuropathic pain following peripheral nerve injury. P2Y12 has seven transmembrane, four cytoplasmic, and four extracellular domains. The transmembrane helices are somewhat tilted and/or kinked. Agonist binding promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. Mutations in P2RY12 gene have been linked to bleeding disorders resulting from severe impairment of platelet response to ADP resulting in defective platelet aggregation. (Ref.: Burnstock G. (2009). Curr. Pharm. Des. 15(15);1717-35; Damman, P., et al. (2012). J. Thromb. Thrombolysis. 33(2); 143-153).

Spécificité

This rabbit polyclonal antibody specifically detects P2Y12 receptor. It targets an epitope with in 13 amino acids from the extracellular domain.

Immunogène

Epitope: extracellular domain

KLH-conjugated linear peptide corresponding to 13 amino acids from the internal region of human P2Y purinoreceptor 12.

Application

Anti-P2Y12 Receptor, Cat. No. ABS1487, is a rabbit polyclonal antibody that detects P2Y purinoceptor 12 and has been tested for Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:250-1,000 dilution from a representative lot detected P2Y12 Receptor in human cerebral cortex and human tonsil tissue sections.

Western Blotting Analysis: 1 µg/mL from a representative lot detected P2Y12 Receptor in human platelet cell lysate.

Research Category
Signaling

Qualité

Evaluated by Western Blotting in PC12 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected P2Y12 Receptor in PC12 cell lysate.

Description de la cible

~50 kDa observed; 39.44 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Forme physique

Affinity Purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stockage et stabilité

Stable for 1 year at 2-8°C from date of receipt.

Autres remarques

Concentration: Please refer to lot specific datasheet.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable