ABE460

Anti-dimethyl Histone H3 (Arg2), symmetric Antibody

serum, from rabbit

• Synonym(s): H3R2me2s, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: polyclonal
• Species reactivity: human
• Application: ChIP, DB, IHC, WB
• Citations: 6

Properties

• biological source: rabbit
• Quality Segment: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), rat (based on 100% sequence homology)
• technique(s): ChIP: suitable, dot blot: suitable, immunohistochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_003520
• UniProt accession no.: P68431
• shipped in: wet ice
• target post-translational modification: dimethylation (Arg2)
• Gene Information: human ... HIST1H3F(8968)

Description

General description

Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. The four core histones, H2A, H2B, H3, and H4, assemble into an octamer (2 molecules of each). Subsequently, 146 base pairs of DNA are wrapped around the octamer, forming a nucleosome, the basic subunit of chromatin. Histones are modified post-translationally by the actions of enzymes in both the nucleus and cytoplasm. These modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). The modifications occur predominantly on the N-terminal and C-terminal tails that extend beyond the nucleosome core particle.

~17 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Immunogen

Epitope: Methylated Arg2

Linear peptide corresponding to human Histone H3 symmetrically dimethylated at Arg2.

Application

Dot Blot (Specificity) Analysis: A 1:1,000 dilution from a representative lot detected Histone H3 (Arg2) in an Absurance Histone H3 Specificity Array (Cat. No. 16-667).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Histone H3 (Arg2) in drosophila chromosomes (Migliori, V., et al. (2012). Nat Struct Mol Biol. 19(2):136-144.).

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Histone H3 (Arg2) from P493-6 cell lysate (Migliori, V., et al. (2012). Nat Struct Mol Biol. 19(2):136-144.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

This Anti-dimethyl Histone H3 (Arg2) antibody, symmetric is validated for use in western blotting, dot blot, IHC & ChIP for the detection of dimethyl Histone H3 (Arg2).

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in untreated and nocodazole treated HeLa cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected dimethyl Histone H3 (Arg2) in 10 µg of nocodazole treated HeLa cell lysate and demonstrated a loss of signal in untreated HeLa cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1