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574797

Sigma-Aldrich

TAE Buffer, 10X, Molecular Biology Grade

A 10X concentrate that can be diluted to a 1X solution containing 40 mM Tris, 40 mM acetate, and 1 mM EDTA, pH ~8.3.

Synonyme(s) :

Tris-Acetate-EDTA Buffer

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About This Item

Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

Forme

liquid

Fabricant/nom de marque

Calbiochem®

Conditions de stockage

OK to freeze
desiccated

Technique(s)

electrophoresis: suitable

Activité étrangère

DNase, RNase, protease, none detected

Conditions d'expédition

ambient

Température de stockage

15-25°C

Description générale

Tris, acetate, and ethylenediaminetetraacetic acid (TAE) buffer consists of tris(2-amino-2-[hydroxymethyl]-1,3-propanediol) base, acetate, and ethylenediaminetetraacetic acid (EDTA). This is one of the most used running buffers in nucleic acid electrophoresis. TAE buffer improves the separation or resolution of large DNA fragments.TAE, a standard buffer, is mainly used to perform agarose gel electrophoresis of DNA and RNA in slab gels. TAE is one of the commonly used buffers in every biochemistry and molecular biology laboratory.

Application

TAE Buffer, 10X, Molecular Biology Grade - Calbiochem has been used in 1X concentration for the preparation of agarose gel and as a running buffer in gel electrophoresis.

Avertissement

Toxicity: Irritant (B)

Notes préparatoires

Dilute 1:10 with H₂O to prepare a 1X solution.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictogrammes

Exclamation mark
GHS07

Mention d'avertissement

Warning

Mentions de danger

H319

Classification des risques

Eye Irrit. 2

Code de la classe de stockage

12 - Non Combustible Liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

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Nature protocols, 13(5), 899-914 (2018-04-07)
Cpf1, a CRISPR endonuclease discovered in Prevotella and Francisella 1 bacteria, offers an alternative platform for CRISPR-based genome editing beyond the commonly used CRISPR-Cas9 system originally discovered in Streptococcus pyogenes. This protocol enables the design of engineered CRISPR-Cpf1 components, both
Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis
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Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric

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