1.00439

ChromoCult^® Listeria Agar Enrichment-Supplement

ChromoCult^®, selective for Listeria spp.

• Synonyme(s) : Listeria Enrichment Supplement
• Code UNSPSC : 41106212
• Nomenclature NACRES : NA.74

Propriétés

• Agence: APHA; ISO 11133; ISO 11290-1; ISO 11290-2
• Niveau de qualité: 100
• Forme: lyophilized powder
• Fabricant/nom de marque: ChromoCult^®
• Technique(s): microbiological culture: suitable
• pH: 6.6 (20 °C in H₂O)
• Application(s): food and beverages; microbiology; pathogen testing
• Température de stockage: 2-8°C
• Adéquation: selective for Listeria spp. (Agar)

Description

Description générale

ChromoCult^® Listeria Agar Enrichment-Supplement is a homogeneous, sterile suspension of L-α-phosphatidylinositol in distilled water. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.

Application

ChromoCult^® Listeria Agar Enrichment-Supplement is used as an additive for the preparation of ChromoCult^® Listeria Selective Agar for detection and enumeration of Listeria monocytogenes and of Listeria sp. in a variety of food products.

Liaison

Replaces: 1004390017

Remarque sur l'analyse

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 Std. 37°C aerobic; other strains at least 48 hrs.

Informations légales

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): WGK 1
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable